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ZAP70 ELISA Kit

ZAP70 Reactivity: Human pTyr493 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2747924
  • Target See all ZAP70 ELISA Kits
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Binding Specificity
    pTyr493, total
    Reactivity
    • 10
    • 5
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human Phospho-ZAP70 (Y493) and Total ZAP70 ELISA Kit. This assay semi-quantitatively measures phophorylated ZAP70 (Tyr493) and Total ZAP70 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human ZAP70 phosphorylated at site Tyrosine-493 and Total ZAP70.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all ZAP70 ELISA Kits
    ZAP70 (zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70))
    Alternative Name
    ZAP70 (ZAP70 Products)
    Synonyms
    SRK ELISA Kit, STCD ELISA Kit, STD ELISA Kit, TZK ELISA Kit, ZAP-70 ELISA Kit, 70kDa ELISA Kit, AI327364 ELISA Kit, Srk ELISA Kit, mrtle ELISA Kit, mur ELISA Kit, zap70 ELISA Kit, MGC80670 ELISA Kit, MGC88962 ELISA Kit, ZAP70 ELISA Kit, zgc:110383 ELISA Kit, zeta chain of T-cell receptor associated protein kinase 70 ELISA Kit, putative tyrosine-protein kinase zap-70 ELISA Kit, zeta-chain (TCR) associated protein kinase ELISA Kit, zeta chain of T cell receptor associated protein kinase 70 ELISA Kit, zeta-chain (TCR) associated protein kinase 70kDa ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa L homeolog ELISA Kit, zeta chain of T cell receptor associated protein kinase 70kDa ELISA Kit, tyrosine-protein kinase ZAP-70 ELISA Kit, ZAP70 ELISA Kit, Smp_149470 ELISA Kit, Zap70 ELISA Kit, zap70.L ELISA Kit, zap70 ELISA Kit, LOC707555 ELISA Kit, LOC100342021 ELISA Kit, LOC100390922 ELISA Kit, LOC100542877 ELISA Kit
    Background
    Zeta-chain associated protein kinase 70 (ZAP70) phosphorylated at Tyrosine-493 and Total ZAP70
    Gene ID
    22637
    UniProt
    P43403
    Pathways
    TCR Signaling, Ubiquitin Proteasome Pathway
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