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CXCL10 ELISA Kit

CXCL10 Reactivity: Human, Rhesus Monkey Colorimetric Sandwich ELISA 20-5000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN2748281
  • Target See all CXCL10 ELISA Kits
    CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
    Reactivity
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rhesus Monkey
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    20-5000 pg/mL
    Minimum Detection Limit
    20 pg/mL
    Application
    ELISA
    Purpose
    Rhesus Monkey IP-10 (CXCL10) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair detects Rhesus Monkey and human IP-10. Other species not determined.
    Sensitivity
    20 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all CXCL10 ELISA Kits
    CXCL10 (Chemokine (C-X-C Motif) Ligand 10 (CXCL10))
    Alternative Name
    IP-10 (CXCL10 Products)
    Synonyms
    CXCL10 ELISA Kit, C7 ELISA Kit, IFI10 ELISA Kit, INP10 ELISA Kit, IP-10 ELISA Kit, SCYB10 ELISA Kit, crg-2 ELISA Kit, gIP-10 ELISA Kit, mob-1 ELISA Kit, CRG-2 ELISA Kit, IP10 ELISA Kit, Ifi10 ELISA Kit, Scyb10 ELISA Kit, C-X-C motif chemokine ligand 10 ELISA Kit, C-X-C motif chemokine 10 ELISA Kit, chemokine (C-X-C motif) ligand 10 ELISA Kit, CXCL10 ELISA Kit, cxl10 ELISA Kit, Cxcl10 ELISA Kit
    Background
    C-X-C motif chemokine 10 (10 kDa interferon gamma-induced protein) (Gamma-IP10) (IP-10) (Small-inducible cytokine B10)
    Gene ID
    574243
    UniProt
    Q8MIZ1
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