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Neurexin 1 ELISA Kit

NRXN1 Reactivity: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN2748415
  • Target See all Neurexin 1 (NRXN1) ELISA Kits
    Neurexin 1 (NRXN1)
    Reactivity
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Rat Neurexin-1 alpha (NRXN1a) ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Rat Neurexin-1 alpha. Other species not yet determined.
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all Neurexin 1 (NRXN1) ELISA Kits
    Neurexin 1 (NRXN1)
    Alternative Name
    Neurexin-1 alpha (NRXN1 Products)
    Synonyms
    Hs.22998 ELISA Kit, PTHSL2 ELISA Kit, SCZD17 ELISA Kit, CG7050 ELISA Kit, CT21808 ELISA Kit, Dmel\\CG7050 ELISA Kit, Nrx ELISA Kit, Nrx1 ELISA Kit, dnrx ELISA Kit, dnrx-1 ELISA Kit, nrx ELISA Kit, Nrxn1b ELISA Kit, NRXN1 ELISA Kit, Nrx-1 ELISA Kit, GB13455 ELISA Kit, GB18754 ELISA Kit, 1700062G21Rik ELISA Kit, 9330127H16Rik ELISA Kit, A230068P09Rik ELISA Kit, mKIAA0578 ELISA Kit, neurexin 1 ELISA Kit, Neurexin 1 ELISA Kit, neurexin I ELISA Kit, neurexin 1b ELISA Kit, neurexin 1a ELISA Kit, NRXN1 ELISA Kit, Nrx-1 ELISA Kit, Nrxn1 ELISA Kit, nrxn1 ELISA Kit, nrxn1b ELISA Kit, nrxn1a ELISA Kit
    Background
    Neurexin-1 (Neurexin I-alpha) (Neurexin-1-alpha)
    Gene ID
    60391
    UniProt
    Q63372
    Pathways
    Synaptic Membrane, Skeletal Muscle Fiber Development
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