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RAF1 ELISA Kit

RAF1 Reactivity: Human, Mouse, Rat pSer301 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Catalog No. ABIN2748581
  • Target See all RAF1 ELISA Kits
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Binding Specificity
    pSer301, total
    Reactivity
    • 8
    • 7
    • 7
    • 1
    • 1
    Human, Mouse, Rat
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Human/Mouse/Rat Phospho-Raf-1 (Ser301) and Total Raf-1 ELISA Kit. This assay semi-quantitatively measures phophorylated Raf-1 (Ser301) and Total Raf-1 in lysate samples.
    Sample Type
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibody pair provided in this kit recognizes human, mouse, and rat Raf-1 phosphorylated at site Serine-301 and Total Raf-1.
    Characteristics
    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product RAF1 ELISA Kit
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Expiry Date
    6 months
  • Target See all RAF1 ELISA Kits
    RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
    Alternative Name
    Raf-1 (RAF1 Products)
    Synonyms
    CRAF ELISA Kit, NS5 ELISA Kit, Raf-1 ELISA Kit, c-Raf ELISA Kit, c-raf ELISA Kit, craf ELISA Kit, raf1 ELISA Kit, RAF1 ELISA Kit, 6430402F14Rik ELISA Kit, AA990557 ELISA Kit, BB129353 ELISA Kit, Craf1 ELISA Kit, D830050J10Rik ELISA Kit, v-Raf ELISA Kit, raf ELISA Kit, cRaf ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase a ELISA Kit, v-raf-leukemia viral oncogene 1 ELISA Kit, Raf-1 proto-oncogene, serine/threonine kinase S homeolog ELISA Kit, RAF1 ELISA Kit, Raf1 ELISA Kit, raf1a ELISA Kit, raf1 ELISA Kit, raf1.S ELISA Kit
    Background
    RAF proto-oncogene serine/threonine-protein kinase (Raf-1) phosphorylated at Serine-301 and Total 301
    Gene ID
    5894
    UniProt
    P04049
    Pathways
    MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling
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