RPS6 ELISA Kit (Ribosomal Protein S6)

Details for Product RPS6 ELISA Kit No. ABIN2748617, Supplier: Log in to see
Antigen
  • (Rp)S6
  • CG10944
  • DS6
  • Dmel\\CG10944
  • M(1)7BC
  • M(1)7C
  • RPS6
  • Rp S6
  • Rps6
  • S6
  • air8
  • air[8]
  • anon-WO02059370.61
  • hen
  • l(1)air8
  • l(1)air[8]
  • l(1)hen
  • pp30
  • rpS6
  • rps6
  • S6R
  • rps6a
  • Ribosomal protein S6
  • 30S ribosomal protein S6
  • Protein RPS-6
  • ribosomal protein S6
  • S6 ribosomal protein
  • RpS6
  • rps6
  • rps-6
  • RPS6
  • Rps6
  • rps6-a
  • LOC100533219
  • LOC100135859
Alternatives
Human RPS6 ELISA Kit
Epitope
pSer235, pSer236
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
11
9
9
1
1
1
1
1
1
1
1
1
Method Type
Cell ELISA
Application
ELISA
Options
Supplier
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Supplier Product No.
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Purpose Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
Sample Type Cell Culture Cells
Analytical Method Semi-Quantitative
Detection Method Colorimetric
Specificity The antibodies provided in this kit recognizes human/mouse/rat RPS6 phosphorylated at Serine-235 / Serine-236 and total RPS6 for comparison.
Characteristics
  • Site and signal pathway-specific
  • In vitro detection of adherent cell culture
  • No sample lysis needed
  • Compatible with a standard ELISA plate reader
  • Faster results than with ELISA
  • Adaptable for high-throughput screening and drug discovery
Components
  • uncoated 96-well Microplate
  • Wash Buffer A
  • Wash Buffer B
  • Fixing Solution
  • Quenching Buffer
  • Blocking Buffer
  • Anti-phospho antibody
  • Anti-pan antibody
  • HRP-Conjugated Secondary Antibody
  • TMB One-Step Substrate
  • Stop Solution
  • Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Alternative Name RPS6 (RPS6 ELISA Kit Abstract)
    Background Disease resistance protein RPS6, Resistance to Pseudomonas syringae 6 (RPS6)
    Gene ID 6194
    UniProt P62753
    Research Area Chromatin and Nuclear Signaling, DNA/RNA, Apoptosis/Necrosis, Translation Factors
    Pathways Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
    Sample Volume 100 μL
    Plate Uncoated
    Protocol
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions For Research Use only
    Storage -20 °C
    Storage Comment The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Expiry Date 6 months
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