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RPS6 ELISA Kit

RPS6 Reactivity: Human, Mouse pSer235 Colorimetric Cell ELISA Cell Culture Cells
Catalog No. ABIN2748617
  • Target See all RPS6 ELISA Kits
    RPS6 (Ribosomal Protein S6 (RPS6))
    Binding Specificity
    pSer235, pSer236
    Reactivity
    • 7
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Detection Method
    Colorimetric
    Method Type
    Cell ELISA
    Application
    ELISA
    Purpose
    Cell-Based Human/Mouse RPS6 (S235/S236) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    Sample Type
    Cell Culture Cells
    Analytical Method
    Semi-Quantitative
    Specificity
    The antibodies provided in this kit recognizes human/mouse/rat RPS6 phosphorylated at Serine-235 / Serine-236 and total RPS6 for comparison.
    Characteristics
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    Components
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Material not included
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Sample Volume
    100 μL
    Plate
    Uncoated
    Protocol
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Assay Procedure

    Seed 10,000-30,000 cells into each well and incubate overnight.Apply various treatment, inhibitors or activators according to manufacture's instructions.Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Expiry Date
    6 months
  • Target See all RPS6 ELISA Kits
    RPS6 (Ribosomal Protein S6 (RPS6))
    Alternative Name
    RPS6 (RPS6 Products)
    Synonyms
    S6 ELISA Kit, rps6 ELISA Kit, rps6a ELISA Kit, S6R ELISA Kit, (Rp)S6 ELISA Kit, CG10944 ELISA Kit, DS6 ELISA Kit, Dmel\\CG10944 ELISA Kit, M(1)7BC ELISA Kit, M(1)7C ELISA Kit, RPS6 ELISA Kit, Rp S6 ELISA Kit, Rps6 ELISA Kit, air8 ELISA Kit, air[8] ELISA Kit, anon-WO02059370.61 ELISA Kit, hen ELISA Kit, l(1)air8 ELISA Kit, l(1)air[8] ELISA Kit, l(1)hen ELISA Kit, pp30 ELISA Kit, rpS6 ELISA Kit, wu:fa92e06 ELISA Kit, wu:fb64g06 ELISA Kit, zgc:92237 ELISA Kit, ribosomal protein S6 ELISA Kit, ribosomal protein S6 S homeolog ELISA Kit, S6 ribosomal protein ELISA Kit, Ribosomal protein S6 ELISA Kit, 30S ribosomal protein S6 ELISA Kit, 40S ribosomal protein S6 ELISA Kit, RPS6 ELISA Kit, rps6.S ELISA Kit, LOC100135859 ELISA Kit, Rps6 ELISA Kit, RpS6 ELISA Kit, rps6 ELISA Kit, rps-6 ELISA Kit, LOC100533219 ELISA Kit
    Background
    Disease resistance protein RPS6, Resistance to Pseudomonas syringae 6 (RPS6)
    Gene ID
    6194
    UniProt
    P62753
    Pathways
    Carbohydrate Homeostasis, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly
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