ADIPOQ ELISA Kit

Catalog No. ABIN2859334

Product Details ADIPOQ ELISA Kit

Target: ADIPOQ (Adiponectin (ADIPOQ))

Binding Specificity: AA 19-244

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10.000 pg/mL

Minimum Detection Limit: 156 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat Adiponectin

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Tissue Homogenate, Urine

Analytical Method: Quantitative

Specificity: Expression system for standard: E.coli
Immunogen sequence: I19-N244

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: E.coli
Immunogen sequence: I19-N244

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plate: Pre-coated

Protocol: rat Adiponectin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Adiponectin has been precoated onto 96-well plates. Standards(E.coli, I19-N244) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Adiponectin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat Adiponectin amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Adiponectin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum, plasma(heparin, EDTA), tissue homogenates or urine to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each rat Adiponectin standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 0.6, Standard deviation: 0.027, CV(%): 4.5
• Sample 2: n=16, Mean(ng/ml): 3.5, Standard deviation: 0.112, CV(%): 3.2
• Sample 3: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.261, CV(%): 3.9,
• Sample 1: n=24, Mean(ng/ml): 1.2, Standard deviation: 0.114, CV(%): 9.5
• Sample 2: n=24, Mean(ng/ml): 3.9, Standard deviation: 0.277, CV(%): 7.1
• Sample 3: n=24, Mean(ng/ml): 5.7, Standard deviation: 0.49, CV(%): 8.6

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for ADIPOQ ELISA Kit (ABIN2859334)

El Tabaa, Sokkar, Ramadan, Abd El Salam, Zaid: "Neuroprotective role of Ginkgo biloba against cognitive deficits associated with Bisphenol A exposure: An animal model study." in: Neurochemistry international, Vol. 108, pp. 199-212, (2017) (PubMed).

Rasekhi, Karandish, Jalali, Mohammadshahi, Zarei, Saki, Shahbazian: "Phylloquinone supplementation improves glycemic status independent of the effects of adiponectin levels in premonopause women with prediabetes: a double-blind randomized controlled clinical trial." in: Journal of diabetes and metabolic disorders, Vol. 14, Issue 1, pp. 1, (2015) (PubMed).

Wu, Weng, Lai, Tsai, Liu, Lee, Tsai: "Effect of Lactobacillus plantarum Strain K21 on High-Fat Diet-Fed Obese Mice." in: Evidence-based complementary and alternative medicine : eCAM, Vol. 2015, pp. 391767, (2015) (PubMed).

Liu, Liu, Li, Han, Peng, Shen: "Adiponectin is expressed in the pancreas of high-fat-diet-fed mice and protects pancreatic endothelial function during the development of type 2 diabetes." in: Diabetes & metabolism, Vol. 40, Issue 5, pp. 363-72, (2014) (PubMed).

Hatami Zargaran, Salehi, Heydari, Babajafari: "The Effects of 6 Isocaloric Meals on Body Weight, Lipid Profiles, Leptin, and Adiponectin in Overweight Subjects (BMI > 25)." in: International cardiovascular research journal, Vol. 8, Issue 2, pp. 52-6, (2014) (PubMed).

Liu, Gu, Zhao, An, Yang: "Glypican 4 may be involved in the adipose tissue redistribution in high-fat feeding C57BL/6J mice with peroxisome proliferators-activated receptor ? agonist rosiglitazone treatment." in: Experimental and therapeutic medicine, Vol. 8, Issue 6, pp. 1813-1818, (2014) (PubMed).

Chen, Li, Zhang, Li, Sun, Zhang, Feng, Zhu: "Effects of resveratrol on the amelioration of insulin resistance in KKAy mice." in: Canadian journal of physiology and pharmacology, Vol. 90, Issue 2, pp. 237-42, (2012) (PubMed).

Target Details for ADIPOQ

Target: ADIPOQ (Adiponectin (ADIPOQ))

Alternative Name: ADIPOQ (ADIPOQ Products)

Synonyms: ADP ELISA Kit, DCAF9 ELISA Kit, 6720489L24Rik ELISA Kit, 9330209L04Rik ELISA Kit, Adp ELISA Kit, Mamdc1 ELISA Kit, RNSVSP4U ELISA Kit, SVSIV1 ELISA Kit, Svp4 ELISA Kit, ACDC ELISA Kit, ACRP30 ELISA Kit, ADIPQTL1 ELISA Kit, ADPN ELISA Kit, APM-1 ELISA Kit, APM1 ELISA Kit, GBP28 ELISA Kit, apM-1 ELISA Kit, 30kDa ELISA Kit, APN ELISA Kit, Acdc ELISA Kit, Acrp30 ELISA Kit, Ad ELISA Kit, adipo ELISA Kit, apM1 ELISA Kit, ADN ELISA Kit, acrpl ELISA Kit, adipo-b ELISA Kit, adipoql ELISA Kit, zgc:153584 ELISA Kit, adipoq ELISA Kit, WD and tetratricopeptide repeats 1 ELISA Kit, MAM domain containing glycosylphosphatidylinositol anchor 2 ELISA Kit, seminal vesicle secretory protein 4 ELISA Kit, adiponectin, C1Q and collagen domain containing ELISA Kit, adiponectin, C1Q and collagen domain containing, b ELISA Kit, adiponectin, C1Q and collagen domain containing L homeolog ELISA Kit, WDTC1 ELISA Kit, Mdga2 ELISA Kit, Svs4 ELISA Kit, ADIPOQ ELISA Kit, Adipoq ELISA Kit, adipoqb ELISA Kit, adipoq.L ELISA Kit

Background:

Background: Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Synonyms: 30 kDa adipocyte complement-related protein ,Adiponectin, C1Q and collagen domain containing ,Protein Adipoq ,Adipoq ,Acdc ,rCG_36676 ,

Full Gene Name: adiponectin, C1Q and collagen domain containing

Gene ID: 246253

UniProt: Q8K3R4