Histone 3 ELISA Kit
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- Target See all Histone 3 (H3) ELISA Kits
- Histone 3 (H3)
- Binding Specificity
- H3K27ac
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Application
- ELISA
- Purpose
- Histone H3 acetyl Lys27 ELISA to quantitate the level of histone H3 lysine 27 acetylation
- Analytical Method
- Quantitative
- Specificity
- Histone H3 acetyl Lys27 ELISA Kits are tested for sensitivity in detecting acetylated lysine 27 on histone H3 by generating a standard curve using the Recombinant Histone H3 acetyl Lys27 protein provided in the kit.
- Components
- Histone H3 acetyl Lys27 ELISA Kits include a 96-stripwell Histone H3 Capture plate, a primary antibody specific for Histone H3 acetyl Lys27, HRP-conjugated anti-rabbit IgG secondary antibody, Assay Dilution Buffer, 20X Wash Buffer, Developing Solution, Stop Solution, Recombinant Histone H3 acetyl Lys27 positive control protein and plate sealer.
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Histone 3 (H3) ELISA Kit
H3 Reactivity: Human, Mouse, Rat Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
Histone 3 (H3) ELISA KitH3 Reactivity: Human, Mouse acetylated Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Histone 3 (H3) ELISA KitH3 Reactivity: Mouse Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Histone 3 (H3) ELISA KitH3 Reactivity: Human, Mouse acetylated Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
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Recombinant protein standards are generated in duplicate from 0.67 ng - 3.75 ng and assayed in the kit according to the protocol listed in the manual. For lot 09715001, a developing time of 5 minutes generated the protein standard curve in Figure 1. The linear range of this lot was plotted in Figure 2. These results are provided for demonstration only. The linear range may vary depending on the developing time used and should be calculated each time a standard curve is run.
- Assay Time
- 3 h
- Plate
- Pre-coated
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
- Store the Recombinant Histone H3 acetyl Lys27 protein at -80°C. Store the Histone H3 acetyl Lys27 antibody and HRP-conjugated anti-mouse IgG1 at -20°C. All other components should be stored at 4°C. This product is guaranteed for 6 months from date of receipt.
- Expiry Date
- 6 months
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- Target See all Histone 3 (H3) ELISA Kits
- Histone 3 (H3)
- Alternative Name
- Histone h3 (H3 Products)
- Synonyms
- H-3 ELISA Kit, histocompatibility 3 ELISA Kit, H3 ELISA Kit
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