ACTH FLISA Kit
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- Target See all ACTH FLISA Kits
- ACTH (Adrenocorticotropic hormone (ACTH))
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Reactivity
- Human
- Detection Method
- Fluorometric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
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Adrenocorticotropic hormone (ACTH) ELISA Kit
ACTH Reactivity: Mouse Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Human Colorimetric Competition ELISA 1.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Rat Colorimetric Competition ELISA 1.23 pg/mL - 100 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Mouse Colorimetric Sandwich ELISA 18.75 pg/mL - 1200 pg/mL Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Rat Colorimetric Competition ELISA 15.63 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma, Serum
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Mouse Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Rat Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Various Species Colorimetric Competition ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Adrenocorticotropic hormone (ACTH) ELISA KitACTH Reactivity: Dog Colorimetric Competition ELISA 12.35 pg/mL - 1000 pg/mL Plasma, Serum
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Plate
- Pre-coated
- Protocol
- The immunoplate in this kit is pre-coated with a secondary antibody and the nonspecific binding sites are blocked. The secondary antibody can bind to the Fc fragment of the primary antibody (peptide antibody) whose Fab fragment will be competitively bound by both biotinylated peptide and peptide standard or targeted peptide in samples. The biotinylated peptide interacts with streptavidin-horseradish peroxidase (SA-HRP) which catalyzes the substrate. The fluorescence intensity is directly proportional to the amount of biotinylated peptide-SA-HRP complex but inversely proportional to the amount of the peptide in standard solutions or samples. This is due to the competitive binding of the biotinylated peptide with the standard peptide or samples to the peptide antibody (primary antibody). A standard curve of known concentration can be established accordingly. The unknown concentration in samples can be determined by extrapolation to this standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all ACTH FLISA Kits
- ACTH (Adrenocorticotropic hormone (ACTH))
- Alternative Name
- ACTH (ACTH Products)
- Synonyms
- ACTH FLISA Kit, BE FLISA Kit, Beta-LPH FLISA Kit, Clip FLISA Kit, Gamma-LPH FLISA Kit, Npp FLISA Kit, Pomc-1 FLISA Kit, Pomc1 FLISA Kit, alpha-MSH FLISA Kit, alphaMSH FLISA Kit, beta-MSH FLISA Kit, gamma-MSH FLISA Kit, CLIP FLISA Kit, LPH FLISA Kit, MSH FLISA Kit, NPP FLISA Kit, POC FLISA Kit, pro-opiomelanocortin-alpha FLISA Kit, proopiomelanocortin FLISA Kit, Pomc FLISA Kit, POMC FLISA Kit
- Target Type
- Hormone
- Pathways
- Metabolism of Steroid Hormones and Vitamin D, Peptide Hormone Metabolism, Hormone Activity
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