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HMGB1 ELISA Kit

HMGB1 Reactivity: Mouse Colorimetric Sandwich ELISA 46.88 pg/mL - 3000 pg/mL Plasma, Serum
Catalog No. ABIN3163168
  • Target See all HMGB1 ELISA Kits
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Reactivity
    • 6
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    46.88 pg/mL - 3000 pg/mL
    Minimum Detection Limit
    46.88 pg/mL
    Application
    ELISA
    Purpose
    The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of HMG1 in mouse serum, plasma.

    We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity

    This assay has high sensitivity and excellent specificity for detection of High Mobility Group Protein 1 (HMG1).
    No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.

    Cross-Reactivity (Details)
    No significant cross-reactivity or interference between High Mobility Group Protein 1 (HMG1) and analogues was observed.
    Sensitivity
    18.29 pg/mL
    Components
    • Pre-coated, ready to use 96-well strip plate, flat buttom
    • Plate sealer for 96 wells
    • Reference Standard
    • Standard Diluent
    • Detection Reagent A
    • Detection Reagent B
    • Assay Diluent A
    • Assay Diluent B
    • Reagent Diluent (if Detection Reagent is lyophilized)
    • TMB Substrate
    • Stop Solution
    • Wash Buffer (30 x concentrate)
    • Instruction manual
    Featured
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    Discover our top product HMGB1 ELISA Kit
  • Application Notes
    • Limited by the current condition and scientific technology, we cannot completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit.
    • The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
    • Kits from different batches may be a little different in detection range, sensitivity and color developing time.
    • Do not mix or substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
    • Protect all reagents from strong light during storage and incubation. All the bottle caps of reagents should be covered tightly to prevent the evaporation and contamination of microorganism.
    • There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results. Do not remove microtiter plate from the storage bag until needed.
    • Wrong operations during the reagents preparation and loading, as well as incorrect parameter setting for the plate reader may lead to incorrect results. A microplate plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 O.D. or greater at 450 ± 10nm wavelength is acceptable for use in absorbance measurement. Please read the instruction carefully and adjust the instrument prior to the experiment.
    • Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
    • Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
    • Kits from different manufacturers for the same item might produce different results, since we have not compared our products with other manufacturers.
    Comment

    Information on standard material:
    The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

    Information on reagents:
    The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

    Information on antibodies:
    The provided antibodies and their host vary in different kits.

    Sample Volume
    100 μL
    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Mobility Group Protein 1 (HMG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Mobility Group Protein 1 (HMG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Mobility Group Protein 1 (HMG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Mobility Group Protein 1 (HMG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Reagent Preparation
    1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
    2. Standard - Reconstitute the Standard with 1.0 mL of Standard Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). The concentration of the standard in the stock solution is 6,000pg/mL. Firstly dilute the stock solution to 3,000pg/mL and the diluted standard serves as the highest standard (3,000pg/mL). Then prepare 7 tubes containing 0.5 mL Standard Diluent and use the diluted standard to produce a double dilution series. Mix each tube thoroughly before the next transfer. Set up 7 points of diluted standard such as 3,000pg/mL, 1,500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.75pg/mL, 46.88pg/mL, and the last microcentrifuge tube with Standard Diluent is the blank as 0pg/mL.
    3. Detection Reagent A and Detection Reagent B - If lyophilized reconstitute the Detection Reagent A with 150μL of Reagent Diluent, keep for 10 minutes at room temperature, shake gently (not to foam). Briefly spin or centrifuge the stock Detection A and Detection B before use. Dilute them to the working concentration 100-fold with Assay Diluent A and B, respectively.
    4. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
    5. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

    Note:

    1. Making serial dilution in the wells directly is not permitted.
    2. Prepare standards within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
    3. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
    4. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
    5. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
    6. Contaminated water or container for reagent preparation will influence the detection result.
    Assay Precision

    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested 20 times on one plate, respectively.
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Mobility Group Protein 1 (HMG1) were tested on 3 different plates, 8 replicates in each plate.
    CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%

    Restrictions
    For Research Use only
  • Precaution of Use
    The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
    Handling Advice
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition.
    To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
    Storage
    4 °C
    Storage Comment
    • For unopened kit: All the reagents should be kept according to the labels on vials. The Standard, Detection Reagent A, Detection Reagent B and the 96-well strip plate should be stored at -20°C upon receipt while the others should be at 4°C.
    • For opened kit: When the kit is opened, the remaining reagents still need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and reseal along entire edge of zip-seal.
      Note: It is highly recommended to use the remaining reagents within 1 month provided this is within the expiration date of the kit.
    • For ELISA kit, 1 day storage at 37°C can be considered as 2 months at 4°C, which means 3 days at 37°C equaling 6 months at 4°C.
    Expiry Date
    6 months
  • Wang, Zhou, Wang, Sun: "Suppression of high-mobility group box 1 ameliorates xerostomia in a Sjögren syndrome-triggered mouse model." in: Canadian journal of physiology and pharmacology, Vol. 98, Issue 6, pp. 351-356, (2021) (PubMed).

    Hanson, Hernady, Reed, Johnston, Groves, Finkelstein: "Apoptosis Resistance in Fibroblasts Precedes Progressive Scarring in Pulmonary Fibrosis and Is Partially Mediated by Toll-Like Receptor 4 Activation." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 170, Issue 2, pp. 489-498, (2020) (PubMed).

    Kawahara, Goto, Kodama, Luo, Fujiwara-Tani, Mori, Miyagawa, Tanaka, Kodama, Hosoito, Taniguchi, Kuniyasu: "Magnetic Hyperthermia Using Self-Controlled Heating Elements Consisting of Fe-Al Milling Alloy Induces Cancer Cell Apoptosis while Preserving Skeletal Muscle." in: Pathobiology : journal of immunopathology, molecular and cellular biology, Vol. 86, Issue 5-6, pp. 254-262, (2020) (PubMed).

    Adamiak, Cymer, Anusz, Tracz, Ratajczak: "A Novel Evidence That Mannan Binding Lectin (MBL) Pathway of Complement Cascade Activation is Involved in Homing and Engraftment of Hematopoietic Stem Progenitor Cells (HSPCs)." in: Stem cell reviews and reports, Vol. 16, Issue 4, pp. 693-701, (2020) (PubMed).

    Yu, Shi, Yu, Liu, Li, Liu, Wang, Chen: "Inhibition of calpain alleviates coxsackievirus B3-induced myocarditis through suppressing the canonical NLRP3 inflammasome/caspase-1-mediated and noncanonical caspase-11-mediated pyroptosis pathways." in: American journal of translational research, Vol. 12, Issue 5, pp. 1954-1964, (2020) (PubMed).

    Linders, Madhi, Rahman, Mörgelin, Regner, Brenner, Wang, Thorlacius: "Extracellular cold-inducible RNA-binding protein regulates neutrophil extracellular trap formation and tissue damage in acute pancreatitis." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 100, Issue 12, pp. 1618-1630, (2020) (PubMed).

    Lee, Wang, Li, Liu: "Anti-inflammatory effect of cinnamaldehyde and linalool from the leaf essential oil of Cinnamomum osmophloeum Kanehira in endotoxin-induced mice." in: Journal of food and drug analysis, Vol. 26, Issue 1, pp. 211-220, (2019) (PubMed).

    Park, Kim, Kim, Chang: "Luteolin activates ERK1/2- and Ca2+-dependent HO-1 induction that reduces LPS-induced HMGB1, iNOS/NO, and COX-2 expression in RAW264.7 cells and mitigates acute lung injury of endotoxin mice." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 67, Issue 5, pp. 445-453, (2018) (PubMed).

    Kim, Park, Kim, Chang: "Sirt1 S-nitrosylation induces acetylation of HMGB1 in LPS-activated RAW264.7 cells and endotoxemic mice." in: Biochemical and biophysical research communications, Vol. 501, Issue 1, pp. 73-79, (2018) (PubMed).

    Wang, Li, Deng, Liu, He: "Ursolic Acid Ameliorates Inflammation in Cerebral Ischemia and Reperfusion Injury Possibly via High Mobility Group Box 1/Toll-Like Receptor 4/NFκB Pathway." in: Frontiers in neurology, Vol. 9, pp. 253, (2018) (PubMed).

    Qian, Wei, Xu, He, Hua, Li, Hu, Lin, Gong, Meng, Zhou, Teng, Song: "Bone marrow-derived mesenchymal stem cells (BMSCs) repair acute necrotized pancreatitis by secreting microRNA-9 to target the NF-κB1/p50 gene in rats." in: Scientific reports, Vol. 7, Issue 1, pp. 581, (2017) (PubMed).

    Chen, Fang, Li, Chen, Li, Gong, Fang: "Glycyrrhizin ameliorates experimental colitis through attenuating interleukin-17-producing T cell responses via regulating antigen-presenting cells." in: Immunologic research, Vol. 65, Issue 3, pp. 666-680, (2017) (PubMed).

    Chaochao, Lou, Yang, Liu, Hu, Min, Chen, He, Chen: "Macrophage Inflammatory Protein-2 in High Mobility Group Box 1 Secretion of Macrophage Cells Exposed to Lipopolysaccharide." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 42, Issue 3, pp. 913-928, (2017) (PubMed).

    Peng, Liu, Ojcius, Lee, Chen, Huang, Martel, Young: "Mineral particles stimulate innate immunity through neutrophil extracellular traps containing HMGB1." in: Scientific reports, Vol. 7, Issue 1, pp. 16628, (2017) (PubMed).

    Yadav, Rani, Deep, Singh, Palle: "Oxidative Stress in Metabolic Disorders: Pathogenesis, Prevention, and Therapeutics." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 9137629, (2016) (PubMed).

    Yu, Yu, Liu, Yu, Liu, Liu, Su, Jiang, Chen: "Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway." in: SpringerPlus, Vol. 5, pp. 215, (2016) (PubMed).

    Kopecka, Porto, Lusa, Gazzano, Salzano, Pinzòn-Daza, Giordano, Desiderio, Ghigo, De Rosa, Caraglia, Riganti: "Zoledronic acid-encapsulating self-assembling nanoparticles and doxorubicin: a combinatorial approach to overcome simultaneously chemoresistance and immunoresistance in breast tumors." in: Oncotarget, Vol. 7, Issue 15, pp. 20753-72, (2016) (PubMed).

    Liu, Ma, Sun, Li, Wang: "High Mobility Group Box1 Protein Is Involved in Endoplasmic Reticulum Stress Induced by Clostridium difficile Toxin A." in: BioMed research international, Vol. 2016, pp. 4130834, (2016) (PubMed).

    Chen, Li, Khan, Shi, Wang, Zheng, Gong, Fang: "HMGB1 exacerbates experimental mouse colitis by enhancing innate lymphoid cells 3 inflammatory responses via promoted IL-23 production." in: Innate immunity, Vol. 22, Issue 8, pp. 696-705, (2016) (PubMed).

    Wu, Sheng, Xie, Li, Chen, Li, Wang, Xu: "Reduced HMGB 1-Mediated Pathway and Oxidative Stress in Resveratrol-Treated Diabetic Mice: A Possible Mechanism of Cardioprotection of Resveratrol in Diabetes Mellitus." in: Oxidative medicine and cellular longevity, Vol. 2016, pp. 9836860, (2016) (PubMed).

  • Target See all HMGB1 ELISA Kits
    HMGB1 (High Mobility Group Box 1 (HMGB1))
    Alternative Name
    HMG1 (HMGB1 Products)
    Synonyms
    HMG1 ELISA Kit, HMG3 ELISA Kit, SBP-1 ELISA Kit, DEF ELISA Kit, HMG-1 ELISA Kit, Hmg1 ELISA Kit, amphoterin ELISA Kit, p30 ELISA Kit, hmgb1 ELISA Kit, ik:tdsubc_1a5 ELISA Kit, wu:fb23c02 ELISA Kit, xx:tdsubc_1a5 ELISA Kit, zgc:56110 ELISA Kit, zgc:77104 ELISA Kit, hmg-1 ELISA Kit, hmg3 ELISA Kit, sbp-1 ELISA Kit, hmg1 ELISA Kit, HMGB1 ELISA Kit, Ac2-008 ELISA Kit, high mobility group box 1 ELISA Kit, high-mobility group box 1 ELISA Kit, high mobility group box 1a ELISA Kit, high mobility group box 1 L homeolog ELISA Kit, high mobility group protein B1 ELISA Kit, HMGB1 ELISA Kit, Hmgb1 ELISA Kit, hmgb1 ELISA Kit, hmgb1a ELISA Kit, hmgb1.L ELISA Kit, LOC100359149 ELISA Kit
    UniProt
    P63158
    Pathways
    p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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