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- Detection Method
- Colorimetric
- Detection Range
- 1-100 μM
- Minimum Detection Limit
- 1 μM
- Application
- Biochemical Assay (BCA)
- Purpose
- The kit is designed for nitrite determination in biological samples.
- Sample Type
- Plasma
- Specificity
- Specific for nitrite determination in plasma samples.
- Characteristics
- The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
- Components
- Plate and the reagents neccesary to perform the assay.
- Material not included
- Pipettes, reaction tubes, plate reader.
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Tumor Necrosis Factor alpha (TNF alpha) ELISA Kit
TNF alpha Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
High Mobility Group Box 1 (HMGB1) ELISA KitHMGB1 Reactivity: Human Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Plasma, Serum
Lipopolysaccharides (LPS) ELISA KitReactivity: Various Species Colorimetric Competition ELISA 12.35 ng/mL - 1000 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Tumor Necrosis Factor alpha (TNF alpha) ELISA KitTNF alpha Reactivity: Human AA 77-233 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 6 (IL6) ELISA KitIL6 Reactivity: Human Colorimetric Sandwich ELISA 7.8-500 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Vascular Cell Adhesion Molecule 1 (VCAM1) ELISA KitVCAM1 Reactivity: Human Colorimetric Sandwich ELISA 1.563-100 ng/mL Plasma, Serum, Tissue Homogenate
Matrix Metalloproteinase 2 (MMP2) ELISA KitMMP2 Reactivity: Human Colorimetric Sandwich ELISA 0.78 ng/mL - 50 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- Sample Volume
- 50 μL
- Assay Time
- 1 h
- Plate
- Uncoated
- Reagent Preparation
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No reagent preparation is needed.
- Sample Preparation
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Plasma samples may be deproteinized before performing the assay.
- Assay Procedure
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Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.
- Calculation of Results
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Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope
- Restrictions
- For Research Use only
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- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
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