Nitrite Determination Assay Kit

Details for Product No. ABIN3172709, Supplier: Log in to see
Detection Range
1-100 μM
Minimum Detection Limit
1 μM
Application
Biochemical Assay (BCA)
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Purpose The kit is designed for nitrite determination in biological samples.
Sample Type Plasma
Detection Method Colorimetric
Specificity Specific for nitrite determination in plasma samples.
Characteristics The overproduction of nitric oxide may lead to oxidative and nitrosative stress. It has been demonstrated that they enhance the development of a variety of diseases, as well as the ageing process1. Regarding nitrosative stress, high levels of iNOS have be
Components Plate and the reagents neccesary to perform the assay.
Material not included Pipettes, reaction tubes, plate reader.
Sample Volume 50 μL
Assay Time 1 h
Plate Uncoated
Reagent Preparation

No reagent preparation is needed.

Sample Preparation

Plasma samples may be deproteinized before performing the assay.

Assay Procedure

Standard Preparation: Prepare the calibrate in 1 mL tubes following the Table 1 ( see in booklet) . Use ultrapure water as diluent. Performing the assay: 1. Add 50 μL of samples or standards in each well (96-well plate). 2.Add 50 μL of Reagent A in each well. Incubate for 10 minutes protected from light. 3.Add 50 μL of Reagent B in each well. Incubate for 10 minutes protected from light. 4.Read the absorbance at 540 nm within 30 minutes.

Calculation of Results

Determine average absorbance value of each experimental sample. Determine its concentration by comparison to the Nitrite Standard reference curve (Figure 2 see images). Nitrite (μM) = (?A540nm- intercept)/ slope

Restrictions For Research Use only
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
Biochemical Assay (BCA) image for Nitrite Determination Assay Kit (ABIN3172709) Typical standard curve