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APOA1 ELISA Kit

APOA1 Reactivity: Human Colorimetric Sandwich ELISA 3.13-200 ng/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN3198396
  • Target See all APOA1 ELISA Kits
    APOA1 (Apolipoprotein A-I (APOA1))
    Reactivity
    • 9
    • 4
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    3.13-200 ng/mL
    Minimum Detection Limit
    3.13 ng/mL
    Application
    ELISA
    Purpose
    This assay employs the quantitative sandwich enzyme immunoassay technique for quantitative detection.
    Sample Type
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    Apolipoprotein A1 / Apo A1, Human
    Sensitivity
    0.73 ng/mL
    Components
    Plate, Standard, Diluent
    Material not included
    Microplate reader capable of measuring absorbance at 450 nm, with correction wavelength set at 570 nm or 630 nm.
    Pipettes and pipette tips.
    50 μL to 300 μL adjustable multichannel micropipette with disposable tips.
    Multichannel micropipette reservoir.
    Beakers, flasks, cylinders necessary for preparation of reagents.
    Deionized or distilled water.
    Polypropylene test tubes for dilution.
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  • Sample Volume
    100 μL
    Assay Time
    3 - 4 h
    Plate
    Pre-coated
    Restrictions
    For Research Use only
  • Buffer
    Buffer contains: 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Intended for research use only and are not for use in diagnostic or therapeutic procedures.
    Treat all chemicals with caution because they can be potentially hazardous.
    It is recommended that this product is handled only by persons who have been trained in laboratory techniques and in accordance with the principles of good laboratory practice. Wear personal protection equipment such as laboratory coat, safety glasses and gloves.
    Avoid direct contact with skin or eyes. Wash immediately with water in the case of contact with skin or eyes. Avoid contact of skin or mucous membranes with kit reagents or specimens. See material safety data sheet(s) for specific advice.
    Pure water or deionized water must be used for reagent preparation.
    The Stop Solution provided with this kit is an acid solution. Wear personal protection equipment with caution.
    Do not expose kit reagents to strong light during storage and incubation.
    Rubber or disposable latex gloves should be worn while handling kit reagents or specimens.
    Avoid contact of substrate solution with oxidizing agents and metal.
    Avoid splashing or generation of aerosols.
    Use disposable pipette tips and/or pipettes to avoid microbial or cross-contamination of reagents or specimens which may invalidate the test.
    Use clean, dedicated reagent trays for dispensing the conjugate and substrate reagent.
    Exposure to acid inactivates the HRP and antibody conjugate.
    Substrate solution must be warmed to room temperature prior to use.
    Decontaminate and dispose specimens and all potentially contaminated materials as they could contain infectious agents. The preferred method of decontamination is autoclaving for a minimum of 1 hour at 121.5 °C.
    Liquid wastes not containing acid and neutralized waste may be mixed with sodium hypochlorite in volumes such that the final mixture contains 1.0 % sodium hypochlorite. Allow 30 minutes for effective decontamination. Liquid waste containing acid must be neutralized prior to the addition of sodium hypochlorite.
    All reagents including microplate, samples, standards and working solution should be warmed to room temperature before use.
    To obtain accurate results, using adhesive film to seal the plate during incubation is suggested.
    It is recommended that all samples and standards be assayed in duplicate.
    Avoid foaming when mixing or reconstituting solutions containing protein.
    To avoid cross-contamination, use separate reservoirs for each reagent and change pipette tips between each standard, sample and reagent.
    When using an automated plate washer, adding a 30 seconds soak period before washing step and/or rotating the plate between wash steps may improve assay precision.
    When pipetting reagents, maintain a consistent order of addition from well-to-well.
    Keep Substrate solution protected from direct strong light. Substrate Solution should turn to gradations of blue after a proper color development.
    Read absorbance within 30 minutes after adding stop solution.
    Take care not to scratch the inner surface of the microwells.
    Storage
    4 °C
    Storage Comment
    Store kit reagents between 2 and 8 °C. Immediately after use remaining reagents should be returned to cold storage (2 to 8 °C).
    Expiry of the kit and reagents is stated on labels. Expiration date of the kit components can only be guaranteed if the components are stored properly, and if, in case of repeated use of one component, this reagent is not contaminated by the first handling.
    Unopened Kit: Store at 2 - 8 °C (See expiration date on the label).
    Opened/Reconstituted Reagents: Up to 1 month at 2 - 8 °C.
    Reconstituted Standard: Up to 1 month at ≤ -20 °C in a manual defrost freezer. Avoid repeated freeze-thaw cycles.
    Microplate Wells: Up to 1 month at 2 - 8 °C. Return unused strips to the foil pouch containing the desiccant pack, reseal along entire edge to maintain plate integrity. Provided this is within the expiration date of the kit
  • Target See all APOA1 ELISA Kits
    APOA1 (Apolipoprotein A-I (APOA1))
    Alternative Name
    Apolipoprotein A1 / Apo A1 (APOA1 Products)
    Synonyms
    Alp-1 ELISA Kit, Apoa-1 ELISA Kit, Brp-14 ELISA Kit, Ltw-1 ELISA Kit, Lvtw-1 ELISA Kit, Sep-1 ELISA Kit, Sep-2 ELISA Kit, Sep2 ELISA Kit, apoA-I ELISA Kit, apoa ELISA Kit, apoa1 ELISA Kit, cb49 ELISA Kit, wu:fb33f01 ELISA Kit, zgc:103718 ELISA Kit, MGC64335 ELISA Kit, MGC89745 ELISA Kit, Apo-AI ELISA Kit, ApoA-I ELISA Kit, APOA1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, ApoAI ELISA Kit, apolipoprotein A1 ELISA Kit, apolipoprotein A-I ELISA Kit, apolipoprotein A-Ia ELISA Kit, apolipoprotein A-I S homeolog ELISA Kit, apolipoprotein A4 ELISA Kit, APOA1 ELISA Kit, Apoa1 ELISA Kit, apoa1a ELISA Kit, apoa1.S ELISA Kit, apoa1 ELISA Kit, LOC100136573 ELISA Kit, apoa-i ELISA Kit, apoa4 ELISA Kit
    Background
    Apolipoprotein A1 (Apo A1) is the major protein component of high density lipoprotein (HDL) in plasma. Chylomicrons secreted from the intestinal enterocyte also contain Apo A1, but it is quickly transferred to HDL in the bloodstream.
    Gene ID
    335
    NCBI Accession
    NM_000039
    UniProt
    P02647
    Pathways
    Regulation of Lipid Metabolism by PPARalpha, Production of Molecular Mediator of Immune Response, Lipid Metabolism
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