Heat Shock Protein Glycoprotein 96 (HSP gp96) ELISA Kit

Antigen: Heat Shock Protein Glycoprotein 96 (HSP gp96)

Reactivity: Rat (Rattus)

Application: ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN368248

Additional Information

Sample Volume: 50 - 100 µL

Assay Time: 1 - 4.5 h

Plate: Pre-coated 8 x 12 strip plate

Components: Reagent (Quantity): Assay plate (1), Standard (2), Sample Diluent (1x20ml), Biotin-antibody Diluent (1x10ml), HRP-avidin Diluent (1x10ml), Biotin-antibody (1x120μl), HRP-avidin (1x120μl), Wash Buffer (25×concentrate) (1x20ml), TMB Substrate (1x10ml), Stop Solution (1x10ml)

Sensitivity: The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.

Minimum Detection Limit: 0.78 pg/mL

Detection Range: 3.12 pg/mL - 200 pg/mL

Application Details

Reagent Preparation: Bring all reagents to room temperature before use.
1. Wash Buffer If crystals have formed in the concentrate, warm up to room temperature and mix gently until the crystals have completely dissolved. Dilute 20 ml of Wash Buffer Concentrate into deionized or distilled water to prepare 500 ml of Wash Buffer. 5
2. Standard Centrifuge the standard vial at 6000-10000rpm for 30s. Reconstitute the Standard with 1.0 ml of Sample Diluent. This reconstitution produces a stock solution of 200 pg/ml. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions. The undiluted standard serves as the high standard (200 pg/ml). The Sample Diluent serves as the zero standard (0 pg/ml). Prepare fresh for each assay. Use within 4 hours and discard after use.
3. Biotin-antibody Centrifuge the vial before opening. Dilute to the working concentration using Biotin-antibody Diluent(1:100), respectively.
4. HRP-avidin Centrifuge the vial before opening. Dilute to the working concentration using HRP-avidin Diluent(1:100), respectively. Precaution: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Application Notes: Detection wavelength: 450 nm

Handling Advice: The kit should not be used beyond the expiration date on the kit label.Do not mix or substitute reagents with those from other lots or sources.It is important that the Standard Diluent selected for the standard curve be consistent with the samples being assayed.If samples generate values higher than the highest standard, dilute the samples with the appropriate Standard Diluent and repeat the assay. 10Any variation in Standard Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Material not included: Microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm. Pipettes and pipette tips. Deionized or distilled water. Squirt bottle, manifold dispenser, or automated microplate washer. An incubator which can provide stable incubation conditions up to 37°C ± 0.5°C.

Storage: 2-8°C

Storage Comment: Unopened: Store at 2 - 8° C. Do not use past kit expiration date.
Opened: May be stored for up to 1 month at 2 - 8° C. Try to keep coated assay plate in a sealed aluminium foil bag, and avoid the damp.

Expiry Date: Refer to the package label for the expiration date

Restrictions: For Research Use only

Publications

Product: Viana, Steer, Rodrigues: "Amyloid-β Peptide-Induced Secretion of Endoplasmic Reticulum Chaperone Glycoprotein GRP94." in: Journal of Alzheimer's disease : JAD, 2011 (PubMed).