Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Florfenicol ELISA Kit

Reactivity: Chemical Colorimetric Competition ELISA
Catalog No. ABIN400594
  • Target
    Florfenicol
    Reactivity
    Chemical
    Detection Method
    Colorimetric
    Method Type
    Competition ELISA
    Application
    ELISA
    Purpose
    This test kit is based on the competitive enzyme immunoassay for the detection of Florfenicol in the tissue (chicken, pork), fish, shrimp and honey. The coupling antigens are pre-coated on the micro-well stripes. The Florfenicol in the sample and the conjugate antigens pre-coated on the micro-well stripes compete for the anti-Florfenicol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value has a negative correlation with the Florfenicol concentration in the sample. This value is compared to the standard curve and the Florfenicol concentration is subsequently obtained.
    Analytical Method
    Qualitative and Quantitative
    Components
    Micro-well strips: 12 strips with 8 removable wells each 6 standard solution (1 mL each): 0 ppb, 0.5 ppb, 1.5 ppb, 4.5 ppb, 13.5 ppb and 40.5 ppb, Enzyme conjugate (12 mL) red cap, Antibody working solution (7 mL) blue cap, Substrate A solution (7 mL) white cap, Substrate B solution (7 mL) black cap, Stop solution (7 mL) yellow cap, 20 concentrated washing buffer (40 mL) white cap, 2 concentrated redissolving solution (50 mL) transparent cap
    Material not included
    Equipments: microplate reader, printer, mixer or stomacher, nitrogen-drying device, oscillator, centrifuge, measuring pipets, balance( a reciprocal sensibility of 0.01 g) Micropipettors: single-channel 20-200L and 100-1000 L, and multi-channel 250 L, Reagents: ethyl acetate, N-hexane, deionized water
  • Plate
    Pre-coated
    Protocol
    Sample pre-treatment: Instructions The following points must be dealt with before the pre-treatment of any kind of sample: Only the disposable tips can be used for the experiments and the tips must be changed when used for absorbing different reagents. Before the experiment, each experimental utensil must be clean and should be re-cleaned if necessary, in order to avoid the contamination that interferes with the experimental results. Solution preparation before sample pre-treatment: Solution I :the 2 concentrated redissolving solution is mixed with deionized water at 1:1 (1 mL 2 concentrated redissolving solution +1 mL deionized water), used for sample redissolving. Samples preparation a) shrimp, fish and meat Homogenize the sample, use stomacher or mixer. Weight 3.0 0.05 g of the homogenized sample, add 6 mL of ethyl acetate, shake properly for 10 min, centrifuge at above 4000 r/min at room temperature for 10 min. Take 2 mL of the supernatant (upper layer, equivalent to 1 g sample), blow to dry with nitrogen at 50-60oC. Dissolve the dry residues in 1 mL of the diluted redissolving solution, add 1 mL N-hexane, shake vigorously for 1 min, centrifuge at above 4000 r/min at room temperature for 15 min. Take 50 L of the lower, aqueous phase for analysis. Fold of dilution of the sample: one b) honey 1 Put 20.05 g into centrifugal tube, add 4 mL deionized water. 2 Add 4 mL ethyl acetate, shake upside and down for 10 min. 3 Centrifuge at above 4000 r/min at room temperature for 10 min. 4 Transfer 1 mL of ethyl acetate upper layer (equivalent to 0.5 g sample) to a new vessel, reduce to dryness with nitrogen at 50-60oC. 5 Add 0.5 mL of the diluted redissolving solution. 6 Take 50 L for analysis. Fold of dilution of the sample: one
    Restrictions
    For Research Use only
  • Storage
    4 °C
  • Target
    Florfenicol
    Target Type
    Chemical
You are here:
Support