Kit EnzyBeads Trypsine 50 incl. Magnet

Application: Protein Digestion (PD)

Catalog no.: ABIN410199

Additional Information

Characteristics: EnzyBeads™ Trypsine offers a broad range of applications including protein identification and protein sequencing studies. Trypsin cleaves peptide bonds at the carboxylic side of lysine and arginine residues, creating peptides in the size range required for mass spectrometry analysis. The optimal pH of trypsin is between 7 and 9. EnzyBeads™ Trypsine are able to digest large as well as very small quantities of proteins (e.g few nanograms). A sample version of EnzyBeads™ Trypsine can be provided for testing (10 digestions).

Sensitivity: The lower limit of EnzyBeads Trypsine digestion is dependent upon the peptide detection method available in the laboratory.

Application Details

Protocol: A protocol is provided with the product. This protocole is available in english or french.

Components: EnzyBeads™ Trypsine: 2 vials containing a suspension of magnetic beads with immobilized Trypsin (Trypsin from bovine pancreas, TPCK treated, the treatment with L-1-Tosylamide-2-phenylethyl chloromethyl ketone (TPCK) inhibits the chymotrypsin activity). EnzyBeads™ Digestion Buffer: 2 vials containing Digestion Buffer mainly composed of ammonium carbonate buffer (pH ~ 8, ~ 25 mM). EnzyBeads™ Magnet: Magnet with a high magnetic property whose design is optimized to avoid taking beads when collecting the digestion supernatants.

Material not included: Centrifuge microtubes or all microtubes compatible with the magnet, 5 uL to 200 uL adjustable pipettes, Disposables tips for pipettes, Deionised water (conductivity 18.2 Momega.cm at RT, 0.2 um filtered)

Storage: The entire kit is stable 9 month at 2-8°C before opening.

Restrictions: For Research Use only