Leptin Receptor (LEPR) ELISA Kit

Antigen: Leptin Receptor (LEPR)

Synonyms: OBR, CD295, obr, cd295, ob-rb

Reactivity: Human

Application: ELISA

Catalog no.: ABIN411319

Additional Information

Characteristics: For quantitative detection of human Leptin receptor in sera, plasma, body fluids, tissue lysates or cell culture supernates.

Alternative name: Leptin Receptor

Description: Leptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus.1 Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family.2 Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases.3 Leptin is also involved in the regulation of blood pressure through the leptin receptor.4

Specificity: No detectable cross-reactivity with any other cytokine.

Sensitivity: Sensitivity: < 8 pg/ml. Range: 156pg/ml-10,000pg/ml

Application Details

Principle: This human Leptin receptor ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Human Leptin receptor specific-specific monoclonal antibodies (clone No. 52263) were precoated onto 96-well plates. The human specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Leptin receptor amount of sample captured in plate.

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing. Don

Components: Lyophilized recombinant human LeptinR standard: 100ng/tube

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl, 450

Storage: Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles (Shipped with wet ice.) Four months at 4°C and eight months at -20°C.

Restrictions: For Research Use only

Images

Leptin Receptor (LEPR) ELISA Kit