Nerve Growth Factor ELISA Kit

Catalog No. ABIN411335

Product Details Nerve Growth Factor ELISA Kit

Target: Nerve Growth Factor (NGF)

Binding Specificity: AA 122-241

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.6-1000 pg/mL

Minimum Detection Limit: 15.6 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human NGF/NGF beta

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: S122-A241

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <1pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: S122-A241

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the NGF-beta family.

Plate: Pre-coated

Protocol: human NGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for NGF has been precoated onto 96-well plates. Standards(NSO, S122-A241) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for NGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human NGF amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernatants or serum to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human NGF standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 86.3, Standard deviation: 5.6, CV(%): 6.5
• Sample 2: n=16, Mean(pg/ml): 201.2, Standard deviation: 14.5, CV(%): 7.2
• Sample 3: n=16, Mean(pg/ml): 469, Standard deviation: 34.2, CV(%): 7.3,
• Sample 1: n=24, Mean(pg/ml): 93.7, Standard deviation: 7.1, CV(%): 7.6
• Sample 2: n=24, Mean(pg/ml): 231.6, Standard deviation: 18.8, CV(%): 8.1
• Sample 3: n=24, Mean(pg/ml): 509.2, Standard deviation: 39.2, CV(%): 7.7

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for Nerve Growth Factor ELISA Kit (ABIN411335)

Zhang, Wang, Zhang, Zhao: "Improvement of postoperative cognitive dysfunction and attention network function of patients with ischemic cerebrovascular disease via dexmedetomidine." in: Experimental and therapeutic medicine, Vol. 15, Issue 3, pp. 2968-2972, (2018) (PubMed).

Gamper, Viereck: "Re: Sheng W, Zhang H, Kirschner-Hermanns R. Could urinary nerve growth factor be a biomarker for overactive bladder? A meta-analysis. Neurourol Urodyn. 2017;9999:1-8. doi: 10.1002/nau.23210." in: Neurourology and urodynamics, (2017) (PubMed).

Ogura, Suzuki, Sakuma, Yamauchi, Orita, Miyagi, Ishikawa, Kamoda, Oikawa, Kanisawa, Takahashi, Sakai, Nagamine, Fukuda, Takahashi, Ohtori, Tsuchiya: "Differences in levels of inflammatory mediators in meniscal and synovial tissue of patients with meniscal lesions." in: Journal of experimental orthopaedics, Vol. 3, Issue 1, pp. 7, (2016) (PubMed).

Guo, Sun, Xu, Zhao, Peng, Wang: "Human umbilical cord mesenchymal stem cells promote peripheral nerve repair via paracrine mechanisms." in: Neural regeneration research, Vol. 10, Issue 4, pp. 651-8, (2015) (PubMed).

Zakharyan, Atshemyan, Gevorgyan, Boyajyan: "Nerve growth factor and its receptor in schizophrenia." in: BBA clinical, Vol. 1, pp. 24-9, (2015) (PubMed).

Forsyth, Krishna, Lawn, Valadez, Qu, Fenstermacher, Fournier, Potthast, Chinnaiyan, Gibney, Zeinieh, Barker, Carter, Cooper, Kenchappa: "p75 neurotrophin receptor cleavage by ?- and ?-secretases is required for neurotrophin-mediated proliferation of brain tumor-initiating cells." in: The Journal of biological chemistry, Vol. 289, Issue 12, pp. 8067-85, (2014) (PubMed).

Tan, Xie, Liu, Chen, Zheng, Tong, Tian: "Low-frequency (1 Hz) repetitive transcranial magnetic stimulation (rTMS) reverses A?(1-42)-mediated memory deficits in rats." in: Experimental gerontology, Vol. 48, Issue 8, pp. 786-94, (2013) (PubMed).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF (NGF Products)

Synonyms: Beta-NGF ELISA Kit, HSAN5 ELISA Kit, NGFB ELISA Kit, Ngfb ELISA Kit, ngfb ELISA Kit, ns:zft386 ELISA Kit, xx:zft386 ELISA Kit, zgc:109730 ELISA Kit, beta-NGF ELISA Kit, hsan5 ELISA Kit, beta-ngf ELISA Kit, NGF ELISA Kit, ngf ELISA Kit, nerve growth factor ELISA Kit, nerve growth factor b (beta polypeptide) ELISA Kit, nerve growth factor S homeolog ELISA Kit, NGF ELISA Kit, Ngf ELISA Kit, ngfb ELISA Kit, ngf ELISA Kit, ngf.S ELISA Kit

Background:

Protein Function: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Inhibits metalloproteinase dependent proteolysis of platelet glycoprotein VI (PubMed:20164177). .

Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis. The standard product used in this kit is human 2.5S NGF, which is a dimmer linking with two polypeptide chains of 120 amino acids.

Synonyms: Beta-nerve growth factor,Beta-NGF,NGF,NGFB,

Full Gene Name: Beta-nerve growth factor

Cellular Localisation: Secreted.

Gene ID: 4803

UniProt: P01138

Pathways: Regulation of Cell Size