Beta-Galactosidase Staining Kit

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Antigen
  • CG9092
  • Dmel\\CG9092
  • beta-GAL
  • beta-Gal-1
  • beta-gal
  • gal
  • lacZ-1
  • zgc:110823
  • ECK0341
  • JW0335
  • EBP
  • ELNR1
  • MPS4B
  • AW125515
  • Bge
  • Bgl
  • Bgl-e
  • Bgl-s
  • Bgl-t
  • Bgs
  • Bgt
  • C130097A14Rik
  • BGAL
  • beta-galactosidase-like
  • beta galactosidase
  • galactosidase, beta 1
  • beta-D-galactosidase
  • LOC100056371
  • LOC100624593
  • Gal
  • glb1
  • GLB1
  • lacZ
  • VV2_1327
  • SSP0105
  • lacH
  • ECL_03691
  • Glb1
Reactivity
Mammalian
9
7
5
4
4
3
2
2
1
1
1
1
1
1
Application
Detection (D), Intracellular Staining (ICS), Light Microscopy (LM)
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Sample Type Cell Culture Cells
Detection Method Colorimetric
Specificity The beta -Galactosidase staining kit utilizes X-gal as the substrate.
Characteristics β-Galactosidase Staining Kit: Simple and Easy-to-use kit for β-Galactosidase Staining.
Components Fixative Solution (1X)
X-Gal (150 mg, lyophilized)
Staining Solution (1X)
Staining Supplement (100X)
Alternative Name beta-Galactosidase (GLB1 ELISA Kit Abstract)
Background The LacZ gene from E. coli is one of the most commonly used reporter genes for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes. The LacZ gene encodes the enzyme β-galactosidase, which is very stable, resistant to proteolytic degradation, can utilize a variety of substrates and can be easily assayed in situ. The β-Galactosidase staining kit utilizes X-gal as the substrate.
Research Area Enzymes
Application Notes The β-Galactosidase staining kit utilizes X-gal as the substrate and LacZ gene as the reporter for testing the efficiency of expression vector mediated gene transfer and for studying the regulation of promoters of genes.
Comment

Further details regarding sample type: Cell culture

Restrictions For Research Use only
Storage -20 °C
Storage Comment Store kit at 4 °C or -20 °C, protected from light. Store reconstituted X-gal in -20 °C.
Expiry Date 12 months
Product cited in: Donninger, Calvisi, Barnoud, Clark, Schmidt, Vos, Clark: "NORE1A is a Ras senescence effector that controls the apoptotic/senescent balance of p53 via HIPK2." in: The Journal of cell biology, Vol. 208, Issue 6, pp. 777-89, 2015 (PubMed).

Kim, Braun, Dragoo: "The effect of resveratrol on normal and osteoarthritic chondrocyte metabolism." in: Bone & joint research, Vol. 3, Issue 3, pp. 51-9, 2014 (PubMed).

Takasaka, Araya, Hara, Ito, Kobayashi, Kurita, Wakui, Yoshii, Yumino, Fujii, Minagawa, Tsurushige, Kojima, Numata, Shimizu, Kawaishi, Kaneko, Kamiya, Hirano, Odaka, Morikawa, Nishimura, Nakayama et al.: "Autophagy induction by SIRT6 through attenuation of insulin-like growth factor signaling is involved in the regulation of human bronchial epithelial cell senescence. ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 192, Issue 3, pp. 958-68, 2014 (PubMed).

Lv, Wang, Li, Wang, Li, Lv, Fu, Hu: "Rupatadine protects against pulmonary fibrosis by attenuating PAF-mediated senescence in rodents." in: PLoS ONE, Vol. 8, Issue 7, pp. e68631, 2013 (PubMed).

Hara, Araya, Ito, Kobayashi, Takasaka, Yoshii, Wakui, Kojima, Shimizu, Numata, Kawaishi, Kamiya, Odaka, Morikawa, Kaneko, Nakayama, Kuwano: "Mitochondrial fragmentation in cigarette smoke-induced bronchial epithelial cell senescence." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 305, Issue 10, pp. L737-46, 2013 (PubMed).

Araya, Kojima, Takasaka, Ito, Fujii, Hara, Yanagisawa, Kobayashi, Tsurushige, Kawaishi, Kamiya, Hirano, Odaka, Morikawa, Nishimura, Kawabata, Hano, Nakayama, Kuwano: "Insufficient autophagy in idiopathic pulmonary fibrosis." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 304, Issue 1, pp. L56-69, 2013 (PubMed).

Cao, Wang, Wang, Xu, Liu, Liu: "Aβ-induced senescent retinal pigment epithelial cells create a proinflammatory microenvironment in AMD." in: Investigative ophthalmology & visual science, Vol. 54, Issue 5, pp. 3738-50, 2013 (PubMed).

Misaka, Suzuki, Miyata, Kobayashi, Shishido, Ishigami, Saitoh, Hirose, Kubota, Takeishi: "Deficiency of senescence marker protein 30 exacerbates angiotensin II-induced cardiac remodelling." in: Cardiovascular research, Vol. 99, Issue 3, pp. 461-70, 2013 (PubMed).

Davalos, Kawahara, Malhotra, Schaum, Huang, Ved, Beausejour, Coppe, Rodier, Campisi: "p53-dependent release of Alarmin HMGB1 is a central mediator of senescent phenotypes." in: The Journal of cell biology, Vol. 201, Issue 4, pp. 613-29, 2013 (PubMed).

Hara, Araya, Takasaka, Fujii, Kojima, Yumino, Shimizu, Ishikawa, Numata, Kawaishi, Saito, Hirano, Odaka, Morikawa, Hano, Nakayama, Kuwano: "Involvement of creatine kinase B in cigarette smoke-induced bronchial epithelial cell senescence." in: American journal of respiratory cell and molecular biology, Vol. 46, Issue 3, pp. 306-12, 2012 (PubMed).

Lambiv, Vassallo, Delorenzi, Shay, Diserens, Misra, Feuerstein, Murat, Migliavacca, Hamou, Sciuscio, Burger, Domany, Stupp, Hegi: "The Wnt inhibitory factor 1 (WIF1) is targeted in glioblastoma and has a tumor suppressing function potentially by induction of senescence." in: Neuro-oncology, Vol. 13, Issue 7, pp. 736-47, 2011 (PubMed).

Lemarié, Shbat, Marchesi, Angulo, Deschênes, Blostein, Paradis, Schiffrin: "Mthfr deficiency induces endothelial progenitor cell senescence via uncoupling of eNOS and downregulation of SIRT1." in: American journal of physiology. Heart and circulatory physiology, Vol. 300, Issue 3, pp. H745-53, 2011 (PubMed).

Minagawa, Araya, Numata, Nojiri, Hara, Yumino, Kawaishi, Odaka, Morikawa, Nishimura, Nakayama, Kuwano: "Accelerated epithelial cell senescence in IPF and the inhibitory role of SIRT6 in TGF-β-induced senescence of human bronchial epithelial cells." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 300, Issue 3, pp. L391-401, 2011 (PubMed).

Muthusamy, Andersson, Kim, Butler, Waage, Bergerheim, Gustafsson: "Estrogen receptor ? and 17?-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 50, pp. 20090-4, 2011 (PubMed).

Araya, Cambier, Markovics, Wolters, Jablons, Hill, Finkbeiner, Jones, Broaddus, Sheppard, Barzcak, Xiao, Erle, Nishimura: "Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients." in: The Journal of clinical investigation, Vol. 117, Issue 11, pp. 3551-62, 2007 (PubMed).