Lactate Assay Kit

Details for Product No. ABIN411682
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Target Name (Antigen)
Detection Range 0.001-10 mM
Application
Biochemical Assay (BCA)
Pubmed 11 references available
Catalog no. ABIN411682
Quantity 100 tests
Price
445.50 $   Plus shipping costs $45.00
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  • +1 888 205 9894 (TF)
Sample Type Cell and Tissue Culture Supernatant, Urine, Plasma, Serum, Biological Fluids
Specificity In the Lactate Assay Kit, lactate specifically reacts with a enzyme mix to generate a product, which interacts with lactate probe to produce color (at lambda = 570 nm) and fluorescence (at Ex/Em = 535/587 nm ). The kit provides a convenient means for detecting L(+)-Lactate in biological samples such as in blood circulation, in cells, in culture mediums, in fermentation mediums, etc. There is no need of pretreatment or purification of samples.
Components Lactate Assay Buffer, Lactate Probe (in DMSO), Lactate Enzyme Mix, L(+)-Lactate Standard (100 nM/µL)
Background Abnormal high concentration of lactate has been related to disease states such as diabetes and lactate acidosis, etc. L(+)-Lactate is the major stereo-isomer of lactate formed in human intermediary metabolism and is present in blood. D(-)-Lactate is also present but only at about 1-5 % of the concentration of L(+)-Lactate.
Protocol 1. Standard Curve Preparations: For the colorimetric assay, d ilute the Lactate Standard (MW 90.08) to 1 nM/µL by adding 10 µL of the Lactate Standard to 990 µL of Lactate Assay Buffer, mix well. Add 0, 2, 4, 6, 8, 10 µL into each well individually. Adjust volume to 50 µL/well with Lactate Assay Buffer to generate 0, 2, 4, 6, 8, 10 nM/well of the L(+)- Lactate Standard. For fluorometric assay, dilute the Lactate Standard to 0.1 nM/µL by adding 10 µL of the Lactate Acid to 990 µL of Lactate Assay Buffer, mix well. Then take 20 µL into 180 µL of Lactate Assay Buffer. Mix well. Add 0, 2, 4, 6, 8, 10 µL into each well individually. Adjust volume to 50 µL/well with Lactate Assay Buffer to generate 0, 0.2, 0.4, 0.6, 0.8, 1.0 nM/well of the Lactate Standard.
2. Sample Preparations: Prepare test samples in 50 µL/well with Lactate Assay Buffer in a 96-well plate. If using serum sample, serum (0.5-10 µL/assay, serum contains approx. 0.6 nM/µL lactate) can be directly diluted in the Lactate Assay Buffer. We suggest using several doses of your sample to ensure the readings are within standard curve range. Note: Lactate Dehydrogenase (LDH) may degrade lactate. Therefore, the samples contain LDH (such as culture medium or tissue lysate) should be kept -80C for storage, or filter samples through 10 Kd molecular weight spin filter.
3. Reaction Mix Preparation: Mix enough reagent for the number of assays performed: For each well, prepare a total 50 µL Reaction Mix containing the following components. Mix well before use. 46 µL Lactate Assay Buffer 2 µL Probe 2 µL Enzyme Mix
4. Add 50 µL of the Reaction Mix to each well containing the Lactate Standard or test samples, mix well.
5. Incubate the reaction for 30 minutes at room temperature, protect from light.
6. Measure O.D. 570 nm for colorimetric assay or fluorescence at Ex/Em = 535/590 nm in a microplate reader. If the background is too high in the fluorometric assay, 1/10 volume of probe may be used, which will decrease the background significantly.
7. Correct background by subtracting the value derived from the 0 lactate control from all sample readings
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Product cited in: Pistollato, Abbadi, Rampazzo et al.: "Hypoxia and succinate antagonize 2-deoxyglucose effects on glioblastoma." in: Biochemical pharmacology, Vol. 80, Issue 10, pp. 1517-27, 2010 (PubMed). Further details: Lactate Assay Extracellular lactate measurement was performed using the Lactate Assay Kit

Rey, Luo, Shimoda et al.: "Metabolic reprogramming by HIF-1 promotes the survival of bone marrow-derived angiogenic cells in ischemic tissue." in: Blood, Vol. 117, Issue 18, pp. 4988-98, 2011 (PubMed).

Zhao, Liu, Liu et al.: "Overcoming Trastuzumab Resistance in Breast Cancer by Targeting Dysregulated Glucose Metabolism." in: Cancer research, Vol. 71, Issue 13, pp. 4585-4597, 2011 (PubMed).

Varum, Rodrigues, Moura et al.: "Energy metabolism in human pluripotent stem cells and their differentiated counterparts." in: PLoS ONE, Vol. 6, Issue 6, pp. e20914, 2011 (PubMed).

Nakano, Tsuji, Miki et al.: "Glycolysis Inhibition Inactivates ABC Transporters to Restore Drug Sensitivity in Malignant Cells." in: PLoS ONE, Vol. 6, Issue 11, pp. e27222, 2011 (PubMed).

Lee, Yi, Ku et al.: "A pumpless perfusion cell culture cap with two parallel channel layers keeping the flow rate constant." in: Biotechnology progress, 2012 (PubMed). Further details: For concentration measurement of lactate and ammonia, a lactate assay kit and an ammonia assay kit are used, respectively.

Jiang, Wang: "Caloric restriction reduces edema and prolongs survival in a mouse glioma model." in: Journal of neuro-oncology, 2013 (PubMed).

Nikooie, Rajabi, Gharakhanlu et al.: "Exercise-induced changes of MCT1 in cardiac and skeletal muscles of diabetic rats induced by high-fat diet and STZ." in: Journal of physiology and biochemistry, 2013 (PubMed).

General Burchard, Zhang, Liu et al.: "microRNA-122 as a regulator of mitochondrial metabolic gene network in hepatocellular carcinoma." in: Molecular systems biology, Vol. 6, pp. 402, 2010 (PubMed).

Hayashi, Tanaka, Hori et al.: "Role of mitochondria in the maintenance of platelet function during in vitro storage." in: Transfusion medicine (Oxford, England), 2011 (PubMed).

Lindgren, Natsuhara, Tian et al.: "Loss of pten causes tumor initiation following differentiation of murine pluripotent stem cells due to failed repression of nanog." in: PLoS ONE, Vol. 6, Issue 1, pp. e16478, 2011 (PubMed).

Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
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