ApoBrdU-IHC DNA Fragmentation Assay Kit

Application: Biochemical Assay (BCA)

Catalog no.: ABIN411730

Application Details

Application Notes: Note: Mix only enough DNA Labeling Solution to complete the number of assays prepared per session. The DNA Labeling Solution is active for approximately 24 hours.) 11. Carefully blot the 1X Reaction Buffer from the specimen, taking care not to touch the specimen. Immediately apply 50 µl of Complete Labeling Reaction Mixture (prepared above) onto each specimen except for the control slides which require only 25 µl each (The use of a cover slip at this point assures even distribution of the reaction mixture and prevents evaporation during incubation). 12. Cover the specimen with a piece of Parafilm cut slightly larger than the specimen (HINT: Folding up one corner of the Parafilm cover slip will aid in its application and removal). Place slides in a humid chamber and incubate at 37 o C for 1 to 1.5 hours. NOTE:

Research Area: DNA/RNA

Restrictions: For Research Use only