ApoBrdU Red DNA Fragmentation Kit

Details for Product No. ABIN411731, Supplier: Log in to see
Reactivity
DNA
Application
Detection (D), Flow Cytometry (FACS)
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Purpose Apo-BrdU-Red In Situ DNA Fragmentation Assay Kit provides complete components including positive and negative control cells for conveniently detecting DNA fragmentation by fluorescence microscopy or flow cytometry. The kit utilizes Br-dUTP (bromolated deoxyuridine triphosphate nucleotides) which is more readily incorporated into DNA strand breaks than other larger ligands (e.g., fluorescein, biotin or digoxigenin). The greater incorporation gives rise to brighter signal when the Br-dUTP sites are identified by a Red fluorescence labeled anti-BrdU monoclonal antibody. The assay is suitable for studying apoptosis with GFP transfected cells.
Brand ApoBrdU™
Sample Type Cell Samples
Detection Method Fluorometric
Characteristics Apo-BrdU-RedTM In Situ DNA Fragmentation Assay Kit: Convenient & Reliable Br-dUTP based Assay to Detect DNA Fragmentation by Fluorescence Microscopy or Flow Cytometry. Includes both Positive and Negative Control Cells. Suitable for Studying Apoptosis with GFP Transfected Cells.
Components
  • Positive Control Cells
  • Negative Control Cells
  • Wash Buffer
  • Reaction Buffer
  • TdT Enzymes
  • Br-dUTP
  • Rinse Buffer
  • Anti-BrdU-Red Antibody
  • 7-AAD/RNase Staining Buffer
Background Internucleosomal DNA fragmentation is a hallmark of apoptosis in mammalian cells.
Application Notes Detection method: Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD).
Applications: The TUNEL-based immunohistochemical staining kit provides complete reagents including positive and negative control cells for convenient detection of DNA fragmentation
Comment

Flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD).
Simple procedure, takes ~ 3 hours
Fast and convenient
The assay is sensitive and stable
The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.

Protocol Note: If DNA cell cycle information is not necessary, add 0.5 mL PBS instead and continue with step 12. 11. Incubate the cells in the dark for 30 min at room temperature. 12. Analyze the cells by flow cytometry (Ex/Em = 488/576 nm for BrdU-Red and 488/655 nm for 7-AAD). Cells should be analyzed within 3 hours of staining.
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Supplier Images
Flow Cytometry (FACS) image for ApoBrdU Red DNA Fragmentation Kit (ABIN411731) ApoBrdU Red DNA Fragmentation Kit
Product cited in: Che, Kraft, Selin, Welsh: "Regulatory T cells resist virus infection-induced apoptosis." in: Journal of virology, Vol. 89, Issue 4, pp. 2112-20, 2015 (PubMed).

Walsh, Waters, Fowler, Lin, Cunningham, Brooks, Rehg, Morse, Teitell: "LKB1 inhibition of NF-κB in B cells prevents T follicular helper cell differentiation and germinal center formation." in: EMBO reports, Vol. 16, Issue 6, pp. 753-68, 2015 (PubMed).

Cassano, Dellavalle, Tedesco, Quattrocelli, Crippa, Ronzoni, Salvade, Berardi, Torrente, Cossu, Sampaolesi: "Alpha sarcoglycan is required for FGF-dependent myogenic progenitor cell proliferation in vitro and in vivo." in: Development (Cambridge, England), Vol. 138, Issue 20, pp. 4523-33, 2011 (PubMed).

Kikuchi, Wada, Shimizu, Izumi, Akutsu, Mitsunaga, Noborio-Hatano, Nobuyoshi, Ozawa, Kano, Furukawa: "Histone deacetylases are critical targets of bortezomib-induced cytotoxicity in multiple myeloma." in: Blood, Vol. 116, Issue 3, pp. 406-17, 2010 (PubMed).

Lin, Hou, Lin, Hsu, Sheu, Lai, Chen, Lee Chao, Wan, Tsai: "Puerariae radix isoflavones and their metabolites inhibit growth and induce apoptosis in breast cancer cells." in: Biochemical and biophysical research communications, Vol. 378, Issue 4, pp. 683-8, 2008 (PubMed).