Nuclear, Cytosol Fractionation Kit

Details for Product No. ABIN412485
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Reactivity
Mammalian
Application
Purification (Purif)
Pubmed 21 references available
Quantity 100 tests
Options
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Purpose This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
Sample Type Biological Fluids, Cell Culture Cells, Tissue Samples
Specificity This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
Characteristics Nuclear,Cytosol Fractionation Kit: Rapid & Convenient Kit to Separate Nuclear Fractions from Cytoplasmic Fractions of Mammalian Cells.
Components Cytosol Extraction Buffer A (CEB-A)
Cytosol Extraction Buffer B (CEB-B)
Nuclear Extraction Buffer (NEB)
DTT (1 M)
Protease Inhibitor Cocktail (lyophilized)
Application Notes This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells and tissues The new kit provides a system that maintains the nuclear and cytoplasmic compartments separate and intact. Transcriptional activity/reporter assays/enzyme activity assays/Western blotting and more downstream assays.
Comment


Simple procedure, takes less than 2-3 hours
Fast and convenient
The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic extracts from cells relatively quick with little or no cross-contaminations

Assay Time 2 - 3 h
Protocol Note: Nuclear extract prepared using the above procedure contains proteins in a concentration approx. 1 mg/mL. If higher concentration is desired, the nuclei can be resuspended in less volume of NEB-Mix (such as 20 µL) in Step
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Product cited in: Zhong, Qin, Zhu et al.: "Oxysterol-binding protein-related protein 8 (ORP8) increases sensitivity of hepatocellular carcinoma cells to Fas-mediated apoptosis." in: The Journal of biological chemistry, Vol. 290, Issue 14, pp. 8876-87, 2015 (PubMed).

Zhang, Liang, Xie et al.: "A novel post-translational modification of nucleolin, SUMOylation at Lys-294, mediates arsenite-induced cell death by regulating gadd45α mRNA stability." in: The Journal of biological chemistry, Vol. 290, Issue 8, pp. 4784-800, 2015 (PubMed).

Tristan, Ramos, Shahani et al.: "Role of apoptosis signal-regulating kinase 1 (ASK1) as an activator of the GAPDH-Siah1 stress-signaling cascade." in: The Journal of biological chemistry, Vol. 290, Issue 1, pp. 56-64, 2015 (PubMed).

Wang, Chiu, Neumaier et al.: "Endothelial cell heparanase taken up by cardiomyocytes regulates lipoprotein lipase transfer to the coronary lumen after diabetes." in: Diabetes, Vol. 63, Issue 8, pp. 2643-55, 2014 (PubMed).

Zhang, Wang, Li et al.: "The Chinese herb isolate yuanhuacine (YHL-14) induces G2/M arrest in human cancer cells by up-regulating p21 protein expression through an p53 protein-independent cascade." in: The Journal of biological chemistry, Vol. 289, Issue 10, pp. 6394-403, 2014 (PubMed).

Yunoki, Tabuchi, Hayashi et al.: "Inhibition of polo-like kinase 1 promotes hyperthermia sensitivity via inactivation of heat shock transcription factor 1 in human retinoblastoma cells." in: Investigative ophthalmology & visual science, Vol. 54, Issue 13, pp. 8353-63, 2013 (PubMed).

Stache, Hölsken, Fahlbusch et al.: "Tight junction protein claudin-1 is differentially expressed in craniopharyngioma subtypes and indicates invasive tumor growth." in: Neuro-oncology, Vol. 16, Issue 2, pp. 256-64, 2014 (PubMed).

Wegiel, Gallo, Csizmadia et al.: "Carbon monoxide expedites metabolic exhaustion to inhibit tumor growth." in: Cancer research, Vol. 73, Issue 23, pp. 7009-21, 2013 (PubMed).

Wu, Zheng, Deng et al.: "A genetic polymorphism in lincRNA-uc003opf.1 is associated with susceptibility to esophageal squamous cell carcinoma in Chinese populations." in: Carcinogenesis, Vol. 34, Issue 12, pp. 2908-17, 2013 (PubMed).

Malmgren, Spégel, Danielsson et al.: "Coordinate changes in histone modifications, mRNA levels, and metabolite profiles in clonal INS-1 832/13 β-cells accompany functional adaptations to lipotoxicity." in: The Journal of biological chemistry, Vol. 288, Issue 17, pp. 11973-87, 2013 (PubMed).

Weniger, Rizzatti, Pérez-Galán et al.: "Treatment-induced oxidative stress and cellular antioxidant capacity determine response to bortezomib in mantle cell lymphoma." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 17, Issue 15, pp. 5101-12, 2011 (PubMed).

Wu, Jeng, Lai: "The SUMOylation of matrix protein M1 modulates the assembly and morphogenesis of influenza A virus." in: Journal of virology, Vol. 85, Issue 13, pp. 6618-28, 2011 (PubMed).

Vossenkämper, Marchès, Fairclough et al.: "Inhibition of NF-κB signaling in human dendritic cells by the enteropathogenic Escherichia coli effector protein NleE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 185, Issue 7, pp. 4118-27, 2010 (PubMed).

Yun, Baik, Kang et al.: "Physical interaction of Jab1 with human serotonin 6 G-protein-coupled receptor and their possible roles in cell survival." in: The Journal of biological chemistry, Vol. 285, Issue 13, pp. 10016-29, 2010 (PubMed).

Silbermann, Schneider, Grassmann: "Stimulation of interleukin-13 expression by human T-cell leukemia virus type 1 oncoprotein Tax via a dually active promoter element responsive to NF-kappaB and NFAT." in: The Journal of general virology, Vol. 89, Issue Pt 11, pp. 2788-98, 2008 (PubMed).

Tatsumi, Kudo, Ueshima et al.: "Noninvasive evaluation of hepatic fibrosis using serum fibrotic markers, transient elastography (FibroScan) and real-time tissue elastography." in: Intervirology, Vol. 51 Suppl 1, pp. 27-33, 2008 (PubMed).

Wade, Kawata, Williams et al.: "Methoxyacetic acid-induced spermatocyte death is associated with histone hyperacetylation in rats." in: Biology of reproduction, Vol. 78, Issue 5, pp. 822-31, 2008 (PubMed).

Shrivastav, Kino, Cunningham et al.: "Human immunodeficiency virus (HIV)-1 viral protein R suppresses transcriptional activity of peroxisome proliferator-activated receptor {gamma} and inhibits adipocyte differentiation: implications for ..." in: Molecular endocrinology (Baltimore, Md.), Vol. 22, Issue 2, pp. 234-47, 2008 (PubMed).

Vila-Carriles, Zhou, Bubien et al.: "Participation of the chaperone Hsc70 in the trafficking and functional expression of ASIC2 in glioma cells." in: The Journal of biological chemistry, Vol. 282, Issue 47, pp. 34381-91, 2007 (PubMed).

Tanno, Sakamoto, Miura et al.: "Nucleocytoplasmic shuttling of the NAD+-dependent histone deacetylase SIRT1." in: The Journal of biological chemistry, Vol. 282, Issue 9, pp. 6823-32, 2007 (PubMed).

Yu, Kone: "Hypermethylation of the inducible nitric-oxide synthase gene promoter inhibits its transcription." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46954-61, 2004 (PubMed).

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Catalog No. ABIN412485
418.00 $
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Quantity
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100 tests
418.00 $

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