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Details for Product No. ABIN412485

Nuclear/Cytosol Fractionation Kit

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Application
Immunoprecipitation (IP)
Pubmed 30 references available
Catalog no. ABIN412485
Quantity 100 tests
Price
390.50 $   Plus shipping costs $45.00
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Availability Will be delivered in 2 to 3 Business Days
Sample Type Cell Culture
Specificity This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
Components Cytosol Extraction Buffer A (CEB-A), Cytosol Extraction Buffer B (CEB-B), Nuclear Extraction Buffer (NEB), DTT (1 M), Protease Inhibitor Cocktail (lyophilized)

Application Details

Handling References back to top
Protocol Note: Nuclear extract prepared using the above procedure contains proteins in a concentration approx. 1 mg/mL. If higher concentration is desired, the nuclei can be resuspended in less volume of NEB-Mix (such as 20 µL) in Step
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
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Csepeggi, Jiang, Kojima et al.: "Somatic Cell Plasticity and Niemann-Pick Type C2 Protein: FIBROBLAST ACTIVATION." in: The Journal of biological chemistry, Vol. 286, Issue 3, pp. 2078-87, 2011 (PubMed).

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