Nuclear,Cytosol Fractionation Kit

Details for Product No. ABIN412485
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Reactivity
Mammalian
Application
Separation (Sep)
Pubmed 21 references available
Quantity 100 tests
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Catalog No. ABIN412485
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Purpose Isolates nuclear,cytosol fractions w,o untracentrifugation
Sample Type Cell Culture
Specificity This Nuclear/Cytosol Extraction Kit provides a complete system that enables the separation of nuclear extract from the cytoplasmic fraction of mammalian cells. The optimized reagents and procedures provided with the kit allow separation of nuclear and cytoplasmic fractions quickly with little or no cross-contaminations. The extracted nuclear and cytoplasmic protein fractions are functional and compatible with downstream assays such as transcriptional activity, RNA splicing, gel shift assay, reporter assays, enzyme activity assays, and Western blotting.
Characteristics Nuclear,Cytosol Fractionation Kit: Rapid & Convenient Kit to Separate Nuclear Fractions from Cytoplasmic Fractions of Mammalian Cells.
Components Cytosol Extraction Buffer A (CEB-A)
Cytosol Extraction Buffer B (CEB-B)
Nuclear Extraction Buffer (NEB)
DTT (1 M)
Protease Inhibitor Cocktail (lyophilized)
Comment

Category: Nuclear Isolation
SampleType adds: Fresh (suspension and adherent) and frozen tissues

Protocol Note: Nuclear extract prepared using the above procedure contains proteins in a concentration approx. 1 mg/mL. If higher concentration is desired, the nuclei can be resuspended in less volume of NEB-Mix (such as 20 µL) in Step
Restrictions For Research Use only
Storage -20 °C
Expiry Date 12 months
Product cited in: Delaney, Ho, Morrison et al.: "Dichloroacetate affects proliferation but not survival of human colorectal cancer cells." in: Apoptosis : an international journal on programmed cell death, 2014 (PubMed).

Ding, Zhang, Zhou et al.: "Dietary ellagic acid improves oxidant-induced endothelial dysfunction and atherosclerosis: Role of Nrf2 activation." in: International journal of cardiology, Vol. 175, Issue 3, pp. 508-14, 2014 (PubMed).

Lee, Choi, Kim et al.: "FK506 reduces calpain-regulated calcineurin activity in both the cytoplasm and the nucleus." in: Anatomy & cell biology, Vol. 47, Issue 2, pp. 91-100, 2014 (PubMed).

Takahashi, Chen, Moiseyev et al.: "Two point mutations of RPE65 from patients with retinal dystrophies decrease the stability of RPE65 protein and abolish its isomerohydrolase activity." in: The Journal of biological chemistry, Vol. 281, Issue 31, pp. 21820-6, 2006 (PubMed).

Kirton, Wilkinson, Canfield et al.: "Dexamethasone downregulates calcification-inhibitor molecules and accelerates osteogenic differentiation of vascular pericytes: implications for vascular calcification." in: Circulation research, Vol. 98, Issue 10, pp. 1264-72, 2006 (PubMed).

Kawakami, Ito, Charoensit et al.: "Evaluation of proinflammatory cytokine production induced by linear and branched polyethylenimine/plasmid DNA complexes in mice." in: The Journal of pharmacology and experimental therapeutics, Vol. 317, Issue 3, pp. 1382-90, 2006 (PubMed).

Kalin, Wang, Ackerson et al.: "Increased levels of the FoxM1 transcription factor accelerate development and progression of prostate carcinomas in both TRAMP and LADY transgenic mice." in: Cancer research, Vol. 66, Issue 3, pp. 1712-20, 2006 (PubMed).

Ruchalski, Mao, Li et al.: "Distinct hsp70 domains mediate apoptosis-inducing factor release and nuclear accumulation." in: The Journal of biological chemistry, Vol. 281, Issue 12, pp. 7873-80, 2006 (PubMed).

Mani, Baltzer, Valle et al.: "Low pH-dependent endosomal processing of the incoming parvovirus minute virus of mice virion leads to externalization of the VP1 N-terminal sequence (N-VP1), N-VP2 cleavage, and uncoating of the full-length genome." in: Journal of virology, Vol. 80, Issue 2, pp. 1015-24, 2005 (PubMed).

Ogawa, Hayashi, Tokunou et al.: "Suberoylanilide hydroxamic acid enhances gap junctional intercellular communication via acetylation of histone containing connexin 43 gene locus." in: Cancer research, Vol. 65, Issue 21, pp. 9771-8, 2005 (PubMed).

Hasegawa, Yamada, Komiyama et al.: "Dihydroflavonol BB-1, an extract of natural plant Blumea balsamifera, abrogates TRAIL resistance in leukemia cells." in: Blood, Vol. 107, Issue 2, pp. 679-88, 2006 (PubMed).

Wang, Sampath, Fukuda et al.: "Disruption of the inhibitor of apoptosis protein survivin sensitizes Bcr-abl-positive cells to STI571-induced apoptosis." in: Cancer research, Vol. 65, Issue 18, pp. 8224-32, 2005 (PubMed).

Kanouchi, Matsumoto, Taga et al.: "Nuclear transfer of perchloric acid-soluble protein by endoplasmic reticulum stressors." in: Protein science : a publication of the Protein Society, Vol. 14, Issue 9, pp. 2344-9, 2005 (PubMed).

Ishitsuka, Hideshima, Hamasaki et al.: "Honokiol overcomes conventional drug resistance in human multiple myeloma by induction of caspase-dependent and -independent apoptosis." in: Blood, Vol. 106, Issue 5, pp. 1794-800, 2005 (PubMed).

Kobayashi, Kajino, Takahashi et al.: "53BP2 induces apoptosis through the mitochondrial death pathway." in: Genes to cells : devoted to molecular & cellular mechanisms, Vol. 10, Issue 3, pp. 253-60, 2005 (PubMed).

Mori, Okabayashi, Yamashita et al.: "Nuclear localization of Japanese encephalitis virus core protein enhances viral replication." in: Journal of virology, Vol. 79, Issue 6, pp. 3448-58, 2005 (PubMed).

Tanito, Masutani, Kim et al.: "Sulforaphane induces thioredoxin through the antioxidant-responsive element and attenuates retinal light damage in mice." in: Investigative ophthalmology & visual science, Vol. 46, Issue 3, pp. 979-87, 2005 (PubMed).

Massaad, Portier, Taglialatela: "Inhibition of transcription factor activity by nuclear compartment-associated Bcl-2." in: The Journal of biological chemistry, Vol. 279, Issue 52, pp. 54470-8, 2004 (PubMed).

Tsukamoto, Irie, Nishimura: "B-Raf contributes to sustained extracellular signal-regulated kinase activation associated with interleukin-2 production stimulated through the T cell receptor." in: The Journal of biological chemistry, Vol. 279, Issue 46, pp. 48457-65, 2004 (PubMed).

Kaburagi, Yamashita, Ito et al.: "Insulin-induced cell cycle progression is impaired in chinese hamster ovary cells overexpressing insulin receptor substrate-3." in: Endocrinology, Vol. 145, Issue 12, pp. 5862-74, 2004 (PubMed).

Laduron, Deplus, Zhou et al.: "MAGE-A1 interacts with adaptor SKIP and the deacetylase HDAC1 to repress transcription." in: Nucleic acids research, Vol. 32, Issue 14, pp. 4340-50, 2004 (PubMed).

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