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FractionPREPTM Cell Fractionation Kit

Application
Immunoprecipitation (IP)
2 references available
Catalog no. ABIN412490
Quantity 50 assays
Price 395.00 $   Plus shipping costs $35.00
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Additional Information

Description This FractionPREP Cell Fractionation system is designed to provide reproducible extraction of four subcellular protein fractions (Cytosol, nucleus, membrane/particulate, and cytoskeletal fractions) from a single mammalian sample. The method is fast and simple, needing only 2 hours and no ultracentrifugation involved. All four protein fractions obtained are suitable for many downstream applications such as 1-D or 2-D gel, enzyme activity assays, gel shift assay, and Western blotting.

Application Details

Restrictions For Research Use only

Publications

Publications Salaun, MacDonald, Larralde et al.: "Poly(A)-binding protein 1 partially relocalizes to the nucleus during herpes simplex virus type 1 infection in an ICP27-independent manner and does not inhibit virus replication." in: Journal of virology, Vol. 84, Issue 17, pp. 8539-48, 2010 (PubMed).

Baumgartner: "Theileria annulata promotes Src kinase-dependent host cell polarisation by manipulating actin dynamics in podosomes and lamellipodia." in: Cellular microbiology, 2010 (PubMed).