anti-Endothelin Receptor A (ETA) Antibodies (IgG) ELISA Kit

Antigen: Anti-Endothelin Receptor A (ETA) Antibodies

Epitope: Igg

Catalog no.: ABIN454017

Additional Information

Alternative name: anti-Endothelin Receptor A (ETA) Antibodies (IgG)

Description: Endothelins (ET) are 21-amino acid vasoconstricting peptides produced primarily in the endothelium having a key role in vascular homeostasis. It mediates the effects through G-Protein-coupled receptors, the Endothelin receptors. There are two key receptor types, ETA and ETB. ETA receptors are found in the smooth muscle tissue of blood vessels where they increase vasoconstriction by ET-1. The anti-Endothelin Receptor A Antibody-EIA is designed for the determination of antibodies (IgG) against the Endothelin receptor subtype A in serum and plasma.

Sensitivity: 5 standards between 2.5 U/ml and 40 U/ml cut off:20 U/ml Sample materials:Serum, Plasma Intraassay-Precision:10.01% Interassay-Precision:12.81%

Application Details

Principle: The anti-Endothelin Receptor A-Antibody-EIA is an antibody screening test. Endothelin- receptor A has been pre-coated onto a microtiter plate. During the first incubation the anti-Endothelin receptor A-Antibodies of the samples are immobilised on the plate. The autoantibodies are detected with a POD labeled anti-human IgG antibody. In the following enzymatic substrate reaction the intensity of the colour correlates with the concentration and/ or avidity of anti-Endothelin receptor A-antibody.

Protocol: Incubation of samples/ standards/ controls 100 µl 2 hrs, 4°C. Wash Incubation of detection antibody 100 µl 1 hr, room temperature. Wash Substrate incubation 100 µl/well 20 min, room temperature. Add Stopp solution 100 µl/well. Read at 450 nm

Restrictions: For Research Use. CE-certified for In Vitro Diagnostics (IVD) Use in the European Union

Images

Typical standard curve (Y-axis: Absorption, X-axis: Concentration(µg/ml))