IL12B ELISA Kit

Catalog No. ABIN4881658

Product Details IL12B ELISA Kit

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Cat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.16-40 ng/mL

Minimum Detection Limit: 0.16 ng/mL

Application: ELISA

Purpose: Feline IL-12 p40 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Quantitative

Specificity: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: feline Fas, IFN-gamma, IL-1 beta, IL-2, IL-4, IL-5, IL-8, IL-10, RANTES

Sensitivity: 0.16 ng/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: IL-12p40 (IL12B Products)

Synonyms: CLMF ELISA Kit, CLMF2 ELISA Kit, IL-12B ELISA Kit, NKSF ELISA Kit, NKSF2 ELISA Kit, p40 ELISA Kit, Il-12b ELISA Kit, Il12p40 ELISA Kit, Il-12p40 ELISA Kit, Il12 ELISA Kit, IL-12p40 ELISA Kit, IL-12 ELISA Kit, IL-12 p40 ELISA Kit, il12b ELISA Kit, il12p40.b ELISA Kit, zgc:152789 ELISA Kit, IL-12.p40 ELISA Kit, IL12p40 ELISA Kit, IL12B ELISA Kit, CLMF p40 ELISA Kit, interleukin 12B ELISA Kit, interleukin 12b ELISA Kit, interleukin 12Ba ELISA Kit, IL12B ELISA Kit, Il12b ELISA Kit, il12ba ELISA Kit

Background: Gene Names: IL12B
Protein names: Interleukin-12 subunit beta (IL-12B) (Cytotoxic lymphocyte maturation factor 40 kDa subunit) (CLMF p40) (IL-12 subunit p40)

Gene ID: 768273

UniProt: O02744

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation