Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

DSCAM ELISA Kit

DSCAM Reactivity: Human Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Catalog No. ABIN4882660
  • Target See all DSCAM ELISA Kits
    DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))
    Reactivity
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Custom Human DSCAM ELISA Kit.
    Sample Type
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all DSCAM ELISA Kits
    DSCAM (Down Syndrome Cell Adhesion Molecule (DSCAM))
    Alternative Name
    DSCAM (DSCAM Products)
    Synonyms
    CHD2-42 ELISA Kit, CHD2-52 ELISA Kit, Dscam ELISA Kit, DSCAM ELISA Kit, chd2-42 ELISA Kit, chd2-52 ELISA Kit, dscam ELISA Kit, GB15141 ELISA Kit, 4932410A21Rik ELISA Kit, 43Bc ELISA Kit, CG17800 ELISA Kit, CT39257 ELISA Kit, DScam ELISA Kit, DmDscam ELISA Kit, Dm_2R:13612 ELISA Kit, Dmel\\CG17800 ELISA Kit, Dscam-hv ELISA Kit, Dscam1 ELISA Kit, FBgn0033159 ELISA Kit, Neu1 ELISA Kit, dScam ELISA Kit, l(2)05518 ELISA Kit, l(2)43Bc ELISA Kit, p270 ELISA Kit, DS cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule a ELISA Kit, Down syndrome cell adhesion molecule 1 ELISA Kit, Down syndrome cell adhesion molecule-like protein Dscam2 ELISA Kit, down syndrome cell adhesion molecule ELISA Kit, Down syndrome cell adhesion molecule L homeolog ELISA Kit, DSCAM ELISA Kit, Dscam ELISA Kit, dscam ELISA Kit, dscama ELISA Kit, Dscam1 ELISA Kit, LOC5573626 ELISA Kit, CpipJ_CPIJ006173 ELISA Kit, dscam.L ELISA Kit
    Gene ID
    1826
    UniProt
    O60469
    Pathways
    Regulation of Cell Size
You are here:
Support