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FOXC2 ELISA Kit
FOXC2
Reactivity: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Product Details FOXC2 ELISA Kit
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Target
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FOXC2
(Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))
Reactivity
All reactivities for FOXC2 elisa kits
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Application
ELISA
Purpose
Custom Human FoxC2 ELISA Kit.
Sample Type
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytical Method
Quantitative
Specificity
This ELISA antibody pair recognizes .
Characteristics
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Components
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Material not included
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Application Details
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Sample Volume
100 μL
Plate
Pre-coated
Protocol
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Handling
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Storage
-20 °C
Storage Comment
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date
6 months
Target Details for FOXC2
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Target
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FOXC2
(Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))
Alternative Name
FoxC2 (FOXC2 Products )
Synonyms
FKHL14 ELISA Kit, LD ELISA Kit, MFH-1 ELISA Kit, MFH1 ELISA Kit, Fkh14 ELISA Kit, Hfhbf3 ELISA Kit, Mfh1 ELISA Kit, Fkhl14 ELISA Kit, FOXC2 ELISA Kit, Foxc2 ELISA Kit, XFD-4 ELISA Kit, fd-4 ELISA Kit, fd-4' ELISA Kit, fkh7 ELISA Kit, foxc2b ELISA Kit, foxc2 ELISA Kit, forkhead box C2 ELISA Kit, forkhead box C2 (MFH-1, mesenchyme forkhead 1) ELISA Kit, forkhead box C2 S homeolog ELISA Kit, forkhead box C2 L homeolog ELISA Kit, forkhead box protein C2 ELISA Kit, FOXC2 ELISA Kit, Foxc2 ELISA Kit, foxc2.S ELISA Kit, foxc2.L ELISA Kit, LOC100734751 ELISA Kit
Gene ID
2303
UniProt
Q99958
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