FOXJ1 ELISA Kit

Catalog No. ABIN4882926

Product Details FOXJ1 ELISA Kit

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human FoxJ1 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificity: This ELISA antibody pair recognizes .

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for FOXJ1

Target: FOXJ1 (Forkhead Box J1 (FOXJ1))

Alternative Name: FoxJ1 (FOXJ1 Products)

Synonyms: FOXJ1 ELISA Kit, foxJ1 ELISA Kit, foxJ ELISA Kit, FKHL-13 ELISA Kit, HFH-4 ELISA Kit, Hfh4 ELISA Kit, Hfh-4 ELISA Kit, FKHL13 ELISA Kit, HFH4 ELISA Kit, fj67h07 ELISA Kit, wu:fj67h07 ELISA Kit, zgc:101713 ELISA Kit, foxj1 ELISA Kit, id:ibd5134 ELISA Kit, zgc:153050 ELISA Kit, FoxJ1-A ELISA Kit, FoxJ1a ELISA Kit, foxj1-a ELISA Kit, foxj1-b ELISA Kit, foxj1a ELISA Kit, hfh-4 ELISA Kit, hfh4 ELISA Kit, xFKH5 ELISA Kit, xFoxJ1 ELISA Kit, xfoxj1' ELISA Kit, forkhead transcription factor J1 ELISA Kit, forkhead box J1 ELISA Kit, forkhead box J1b ELISA Kit, forkhead box J1a ELISA Kit, forkhead box J1 S homeolog ELISA Kit, FoxJ1 ELISA Kit, FOXJ1 ELISA Kit, foxj1 ELISA Kit, foxJ1 ELISA Kit, Foxj1 ELISA Kit, foxj1b ELISA Kit, foxj1a ELISA Kit, foxj1.S ELISA Kit

Gene ID: 2302

UniProt: Q92949

Pathways: Regulation of Leukocyte Mediated Immunity