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GATA1 ELISA Kit

GATA1 Reactivity: Human Colorimetric Sandwich ELISA 16.38-4000 pg/mL Cell Culture Supernatant, Plasma, Serum
Catalog No. ABIN4883017
  • Target See all GATA1 ELISA Kits
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Reactivity
    • 7
    • 5
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    16.38-4000 pg/mL
    Minimum Detection Limit
    16.38 pg/mL
    Application
    ELISA
    Purpose
    Human GATA-1/Eryf1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Sample Type
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificity
    This ELISA antibody pair recognizes Human GATA-1.
    Sensitivity
    16 pg/mL
    Characteristics
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Components
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Material not included
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product GATA1 ELISA Kit
  • Application Notes
    Recommended Dilution for serum and plasma samples2 fold
    Sample Volume
    100 μL
    Plate
    Pre-coated
    Protocol
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Storage
    -20 °C
    Storage Comment
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Expiry Date
    6 months
  • Target See all GATA1 ELISA Kits
    GATA1 (GATA Binding Protein 1 (Globin Transcription Factor 1) (GATA1))
    Alternative Name
    GATA-1 (GATA1 Products)
    Synonyms
    ERYF1 ELISA Kit, GATA-1 ELISA Kit, GF-1 ELISA Kit, GF1 ELISA Kit, NF-E1 ELISA Kit, NFE1 ELISA Kit, XLANP ELISA Kit, XLTDA ELISA Kit, XLTT ELISA Kit, Gata-1 ELISA Kit, Gf-1 ELISA Kit, eryf1 ELISA Kit, Eryf1 ELISA Kit, GATA1 ELISA Kit, gata-1 ELISA Kit, Gata1 ELISA Kit, si:dkeyp-77f7.5 ELISA Kit, gata1 ELISA Kit, gata1a-A ELISA Kit, xGATA-1 ELISA Kit, xgata1 ELISA Kit, XGATA-1B ELISA Kit, gata1b-A ELISA Kit, gta1 ELISA Kit, zg1 ELISA Kit, NF-E1a ELISA Kit, GATA binding protein 1 ELISA Kit, GATA binding protein 1 (globin transcription factor 1) ELISA Kit, amidase ELISA Kit, aspartyl/glutamyl-tRNA amidotransferase subunit A ELISA Kit, putative amidase ELISA Kit, GatA1 ELISA Kit, glutamyl-tRNA amidotransferase subunit A ELISA Kit, hematopietic transcription factor GATA-1 ELISA Kit, GATA binding protein 1b ELISA Kit, GATA binding protein 1 L homeolog ELISA Kit, GATA binding protein 1 S homeolog ELISA Kit, GATA binding protein 1a ELISA Kit, erythroid transcription factor ELISA Kit, GATA1 ELISA Kit, Gata1 ELISA Kit, gatA1 ELISA Kit, gata-1 ELISA Kit, gata1b ELISA Kit, gata1.L ELISA Kit, gata1.S ELISA Kit, gata1a ELISA Kit, LOC108348091 ELISA Kit
    Gene ID
    2623
    UniProt
    P15976
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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