IL1A ELISA Kit

Catalog No. ABIN4883338

Product Details IL1A ELISA Kit

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.5-300 pg/mL

Minimum Detection Limit: 0.5 pg/mL

Application: ELISA

Purpose: Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Sample Type: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificity: This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.

Sensitivity: 0.5 pg/mL

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Recommended Dilution for serum and plasma samples2 fold

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

References for IL1A ELISA Kit (ABIN4883338)

Podrini, Schramm, Marianantoni, Apolinarska, McGuckin, Forraz, Milet, Desroches, Payen, DAguanno, Biazzo: "Topical Administration of Lactiplantibacillus plantarum (SkinDuoTM) Serum Improves Anti-Acne Properties." in: Microorganisms, Vol. 11, Issue 2, (2023) (PubMed).

Odewumi, Latinwo, Sinclair, Badisa, Abdullah, Badisa: "Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 6422-6, (2015) (PubMed).

Park, Cheon: "Malignant small bowel obstruction: the last frontier for gastrointestinal stenting." in: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1136-7, (2012) (PubMed).

Goktas, Coskun, Bicik, Horuz, Unsal, Serteser, Albayrak, Sarıca: "Evaluating ESWL-induced renal injury based on urinary TNF-α, IL-1α, and IL-6 levels." in: Urological research, Vol. 40, Issue 5, pp. 569-73, (2012) (PubMed).

Liu, Tsuang, Sheu, Sun, Shih: "The protective effects of coumestrol against amyloid-β peptide- and lipopolysaccharide-induced toxicity on mice astrocytes." in: Neurological research, Vol. 33, Issue 6, pp. 663-72, (2012) (PubMed).

Mamlouk, Balagurumoorthy, Wang, Adelstein, Kassis: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." in: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).

Cuchacovich, Hagan, Khan, Richert, Espinoza: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." in: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).

Meltzer, Standley: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." in: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, (2008) (PubMed).

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL-1 alpha (IL1A Products)

Synonyms: IL-1A ELISA Kit, IL1 ELISA Kit, IL1-ALPHA ELISA Kit, IL1F1 ELISA Kit, IL-6 ELISA Kit, Il-1a ELISA Kit, IL-1 alpha ELISA Kit, IL-1alpha ELISA Kit, L1A ELISA Kit, interleukin 1 alpha ELISA Kit, interleukin 6 ELISA Kit, IL1A ELISA Kit, IL6 ELISA Kit, Il1a ELISA Kit

Background: Gene Names: IL1A IL1F1
Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)

Gene ID: 3552

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints