SULT1C2 ELISA Kit

Catalog No. ABIN4884689

Product Details SULT1C2 ELISA Kit

Target: SULT1C2 (Sulfotransferase Family, Cytosolic, 1C, Member 2 (SULT1C2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human SULT1C2 ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Expiry Date: 6 months

Target Details for SULT1C2

Target: SULT1C2 (Sulfotransferase Family, Cytosolic, 1C, Member 2 (SULT1C2))

Alternative Name: SULT1C2 (SULT1C2 Products)

Synonyms: ST1C1 ELISA Kit, ST1C2 ELISA Kit, SULT1C1 ELISA Kit, humSULTC2 ELISA Kit, 1810008N17Rik ELISA Kit, Sult1c1 ELISA Kit, st1c1 ELISA Kit, st1c2 ELISA Kit, sult1c1 ELISA Kit, MGC88979 ELISA Kit, SULT1C2 ELISA Kit, Sult1c2 ELISA Kit, sulfotransferase family 1C member 2 ELISA Kit, sulfotransferase family, cytosolic, 1C, member 2 ELISA Kit, sulfotransferase family 1C member 2 S homeolog ELISA Kit, sulfotransferase 1C2 ELISA Kit, SULT1C2 ELISA Kit, Sult1c2 ELISA Kit, sult1c2 ELISA Kit, sult1c2.S ELISA Kit, LOC100060302 ELISA Kit, LOC100729651 ELISA Kit, LOC100623541 ELISA Kit

Gene ID: 6819

UniProt: O00338