FAM19A1 ELISA Kit (Family with Sequence Similarity 19 (Chemokine (C-C Motif)-Like), Member A1)

Details for Product FAM19A1 ELISA Kit No. ABIN4884716, Supplier: Log in to see
Antigen
  • TAFA-1
  • TAFA1
  • C630007B19Rik
  • FAM19A1 TAFA-1
  • Tafa-1
  • family with sequence similarity 19 (chemokine (C-C motif)-like), member A1
  • family with sequence similarity 19, member A1
  • FAM19A1
  • Fam19a1
Reactivity
Human
Method Type
Sandwich ELISA
Application
ELISA
Options
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Purpose Custom Human TAFA1 (FAM19A1) ELISA Kit.
Sample Type Body Fluids, Cell Culture Supernatant, Plasma, Serum, Cell and Tissue Lysate
Analytical Method Quantitative
Detection Method Colorimetric
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Alternative Name TAFA1 (FAM19A1 ELISA Kit Abstract)
Gene ID 407738
UniProt Q7Z5A9
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Restrictions For Research Use only
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months
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