CD300a ELISA Kit

Catalog No. ABIN4885159

Product Details CD300a ELISA Kit

Target: CD300a (CD300A)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Custom Human CD300a (LMIR1) SpeedELISA Kit.

Brand: SpeedELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Compressed workflow with only one wash step and three incubation steps
• 3 hours total processing time
• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Streptavidin-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Capture Antibody
• HRP Conjugated Detection Antibody
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Sample Volume: 100 μL

Assay Time: 3 h

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 70 μL of the Antibody Cocktail to each well.
3. Incubate 10 minutes at RT.
4. Add 50 μL of sample or standard to each well.
5. Incubate 2 h at RT.
6. Wash Wells
7. Add 100 μL of TMB One-Step Substrate Reagent to each well.
8. Incubate 30 min at RT.
9. Add 50 μL of Stop Solution to each well.
10. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Opened Microplate Wells or reagents may be store for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Reconstituted standard can be stored at -80°C for up to 1 week.
Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Expiry Date: 6 months

Target Details for CD300a

Target: CD300a (CD300A)

Alternative Name: CD300a (CD300A Products)

Synonyms: CD300L-B1 ELISA Kit, CD300A ELISA Kit, clm8 ELISA Kit, B230315M08Rik ELISA Kit, Clm8 ELISA Kit, LMIR1 ELISA Kit, MAIR-I ELISA Kit, MAIR-Ia ELISA Kit, MMAC8 ELISA Kit, Pigr4 ELISA Kit, mcpir1 ELISA Kit, CLM-8 ELISA Kit, CMRF-35-H9 ELISA Kit, CMRF-35H ELISA Kit, CMRF35-H ELISA Kit, CMRF35-H9 ELISA Kit, CMRF35H ELISA Kit, CMRF35H9 ELISA Kit, IGSF12 ELISA Kit, IRC1 ELISA Kit, IRC1/IRC2 ELISA Kit, IRC2 ELISA Kit, IRp60 ELISA Kit, CD300 molecule like family member g ELISA Kit, CD300a molecule ELISA Kit, CMRF35-like molecule 8 ELISA Kit, CD300A molecule ELISA Kit, Cd300a molecule ELISA Kit, CD300LG ELISA Kit, CD300A ELISA Kit, LOC483289 ELISA Kit, clm8 ELISA Kit, Cd300a ELISA Kit

Gene ID: 11314

UniProt: Q9UGN4

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process