Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

MUC16 CLIA Kit

CA125 Reactivity: Human Chemiluminescent Sandwich ELISA
Catalog No. ABIN504742
  • Target See all MUC16 (CA125) CLIA Kits
    MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
    Reactivity
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Chemiluminescent
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Purpose
    Chemiluminescence Immuno-assay: The essential reagents required for an immunoenzymometric assay include high affinity and specificity antibodies (enzyme and immobilized), with different and distinct epitope recognition, in excess, and native antigen. In this procedure, the immobilization takes place during the assay at the surface of a microplate well through the interaction of streptavidin coated on the well and exogenously added biotinylated monoclonal anti-CA-125 antibody. Upon mixing monoclonal biotinylated antibody, the enzyme-labeled antibody and a serum containing the native antigen, reaction results between the native antigen and the antibodies, without competition or steric hindrance, to form a soluble sandwich complex.
    Analytical Method
    Quantitative
    Characteristics
    The Quantitative Determination of Cancer Antigen 125 (CA-125) Concentration in Human Serum by a Microplate Chemiluminescence Immunoassay
    Featured
    Discover our best selling CA125 ELISA Kit
    Top Product
    Discover our top product CA125 ELISA Kit
  • Application Notes
    All products that contain human serum have been found to be non-reactive for Hepatitis B Surface Antigen, HIV 1&2 and HCV Antibodies by FDA required tests. Since no known test can offer complete assurance that infectious agents are absent, all human serum products should be handled as potentially hazardous and capable of transmitting disease. Good laboratory procedures for handling blood products can be found in the Center for Disease Control / National Institute of Health, "Biosafety in Microbiological and Biomedical Laboratories," 2nd Edition, 1988, HHS Publication No. (CDC) 88-8395.
    Plate
    Pre-coated
    Protocol

    Specimien Collection and Preparation:

    The specimens shall be blood, serum in type and the usual precautions in the collection of venipuncture samples should be observed. For accurate comparison to established normal values, a fasting morning serum sample should be obtained. The blood should be collected in a plain redtop venipuncture tube without additives or anti-coagulants. Allow the blood to clot. Centrifuge the specimen to separate the serum from the cells. Samples may be refrigerated at 2_x001E_8(C for a maximum period of five (5) days. If the specimen(s) cannot be assayed within this time, the sample(s) may be stored at temperatures of _x001E_20(C for up to 6 months. Avoid repetitive freezing and thawing. When assayed in duplicate, 0.050ml of the specimen is required. For disease monitoring purposes paired samples should be used. Samples from the previous draws that were stored frozen and never thawed before should be used. The test kit results can not be inter changed.

    Reagent Preparation:

    1. Wash Buffer Dilute contents of Wash Concentrate to 1000ml with distilled or deionized water in a suitable storage container. Store diluted buffer at room temperature 20-27(C. 2. Working Signal Reagent Solution - Store at 2 - 8(C. Determine the amount of reagent needed and prepare by mixing equal portions of Signal Reagent A and Signal Reagent B in a clean container. For example, add 1 ml of A and 1ml of B per two (2) eight well strips (A slight excess of solution is made). Discard the unused portion if not used within 36 hours after mixing. If complete utilization of the reagents is anticipated, within the above time constraint, pour the contents of Signal Reagent B into Signal Reagent A and label accordingly. _x000E_

    Test Procedure:

    Before proceeding with the assay, bring all reagents, serum references and controls to room temperature (20 - 27(C). 1. Format the microplates wells for each serum reference, control and patient specimen to be assayed in duplicate. Replace any unused microwell strips back into the aluminum bag, seal and store at 2-8(C. 2. Pipette 0.025 ml (25l) of the appropriate serum reference, con_x001F_trol or specimen into the assigned well. 3. Add 0.100 ml (100l) of the CA-125 Tracer Reagent to each well. It is very important to dispense all reagents close to the bottom of the coated well. 4. Swirl the microplate gently for 20-30 seconds to mix and cover. 5. Incubate 45 minutes at room temperature. 6. Discard the contents of the microplate by decantation or aspiration. If decanting, tap and blot the plate dry with absorbent paper. 7. Add 350l of wash buffer (see Reagent Preparation Section), decant (tap and blot) or aspirate. Repeat four (4) additional times for a total of five (5) washes. An automatic or manual plate washer can be used. Follow the manufacturers instruction for proper usage. If a squeeze bottle is employed, fill each well by depressing the container (avoiding air bubbles) to dispense the wash. Decant the wash and repeat four (4) additional times. Add 0.100 ml (100l) of working signal reagent to all wells (see Reagent Preparation Section). ). Always add reagents in the same order to minimize reaction time differences between wells. Incubate for five (5) minutes in the dark. 10. Read the relative light units in each well for 0.2 1.0 seconds. The results should be read within thirty (30) minutes of adding the substrate solution.
    Restrictions
    For Research Use only
  • Target See all MUC16 (CA125) CLIA Kits
    MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))
    Alternative Name
    Cancer Antigen 125 (CA-125) (CA125 Products)
    Synonyms
    CA125 CLIA Kit, 1110008I14Rik CLIA Kit, Ca125 CLIA Kit, Gm1480 CLIA Kit, RGD1308422 CLIA Kit, MUC16 CLIA Kit, mucin 16, cell surface associated CLIA Kit, mucin 16 CLIA Kit, mucin-16 CLIA Kit, MUC16 CLIA Kit, Muc16 CLIA Kit, LOC476718 CLIA Kit, LOC712735 CLIA Kit
    Background
    Cancer Antigen 125 (CA-125) is a glycoprotein that occurs in blood as high molecular weight (Mr > 200,000). High concentrations of this antigen are associated with ovarian cancer and a range of benign and malignant diseases. Although the specificity and sensitivity of CA-125 assays are somewhat limited, especially in early diagnosis of ovarian cancer, the assay has found widespread use in the differential diagnosis of adnexal masses, in monitoring disease progression and response to therapy in ovarian cancer, and in the early detection of recurrence after surgery or chemotherapy for ovarian cancer. Published literature has shown that elevated serum CA-125 levels can be observed in patients with serious endometroid, clear cell and undifferentiated ovarian carcinoma. The serum CA-125 is elevated in 1% of normal healthy women, 3% of normal healthy women with benign ovarian diseases, and 6% of patients with non-neoplastic conditions (including but not limited to first trimester pregnancy, menstruation, endometriosis uterine fibrosis, acute salphingitis, hepatic diseases and inflammation of peritoneum or pericardium). In this method, CA-125 calibrator, patient specimen or control is first added to a streptavidin coated well. Biotinylated monoclonal and enzyme labeled antibodies (directed against distinct and different epitopes of CA-125) are added and the reactants mixed. Reaction between the various CA-125 antibodies and native CA-125 forms a sandwich complex that binds with the streptavidin coated to the well. After the completion of the required incubation period, the enzyme-CA-125 antibody bound conjugate is separated from the unbound enzyme-CA-125 conjugate by aspiration or decantation. The activity of the enzyme present on the surface of the well is quantitated by reaction with a suitable substrate to produce light. The employment of several serum references of known Cancer Antigen 125 (CA-125) levels permits the construction of a dose response curve of activity and con_x001F_centration. From comparison to the dose response curve, an unknown specimen's activity can be correlated with CA-125 concentration.
You are here:
Support