RFP-Trap®

Details for Product No. ABIN509416, Supplier: Log in to see
Antigen
Reactivity
Discosoma
6
1
1
Host
Camelidae
Antibody Type
Recombinant Antibody
Application
Affinity Measurement (AM), Chromatin Immunoprecipitation (ChIP), Enzyme Activity Assay (EAA), Immunoprecipitation (IP), Mass Spectrometry (MS), Protein Complex Immunoprecipitation (Co-IP), Pull-Down Assay (Pull-Down), Purification (Purif)
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Purpose For biochemical analyses of RFP fusion proteins.
Brand RFP-Trap®
Sample Type Cell Extracts
Cross-Reactivity (Details) RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby, mKate2 and RFP-tagged fusion proteins. No cross-reaction to DsRed, mRFPruby, TagRFP and all GFPs can be detected.
Characteristics Antibodies - extremely powerful tools in biomedical research - are large complex molecules (~ 150 kDa) consisting of two heavy and two light chains. Due to their complex structure, the use of antibodies is often limited and hindered by batch-to-batch variations.

Camelidae (camels, dromedaries, llamas and alpacas) possess functional antibodies devoid of light chains, so-called heavy chain antibodies (hcAbs). hcAbs recognize and bind their antigens via a single variable domain (VHH). These VHH domains are the smallest intact antigen binding fragments (~ 13 kDa).

Nano-Traps are based on single domain antibody fragments (VHHs) derived from alpaca.
Purification Purified protein
Components RFP-Trap® uncoupled
Material not included Lysis buffer (CoIP), 10x RIPA buffer, Dilution buffer, Wash buffer, Elution buffer
Alternative Name RFP (RFP ELISA Kit Abstract)
Research Area Tags/Labels
Application Notes Red fluorescent proteins (RFP) and variants thereof are widely used to study protein localization and dynamics. For biochemical analyses including mass spectroscopy and enzyme activity measurements these RFP-fusion proteins and their interacting factors can be isolated fast and efficiently (one step) via immunoprecipitation using the RFP-Trap. The RFPTrap_A enables purification of any protein of interest fused to RFP.
Assay Time 1.5 h
Protocol
  • Robust and versatile tool for biochemical analyses of RFP-fusion proteins
  • Short incubation times (5 - 30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
  • Low unspecifi c binding
  • No contaminating heavy and light chains of conventional antibodies
  • Applicable in Chromatin Immunoprecipitation (ChIP)
Restrictions For Research Use only
Format Liquid
Concentration 250 µL resin (1mg/mL)
Buffer 1 x PBS,0.01% Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Expiry Date 12 months
Supplier Images
 image for RFP-Trap® (ABIN509416) RFP-Trap A
Product cited in: Hellewell, Gong, Schärich, Christofidou, Adams: "Modulation of the Extracellular Matrix Patterning of Thrombospondins by Actin Dynamics and Thrombospondin Oligomer State." in: Bioscience reports, 2015 (PubMed).

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Luo, Xin, Du, Ye, Wei: "Dimerization mediated by a divergent FHA domain is essential for the DNA damage and spindle functions of fission yeast Mdb1." in: The Journal of biological chemistry, 2015 (PubMed).

Hobbs, DePianto, Jacob, Han, Chung, Batazzi, Poll, Guo, Han, Ong, Zheng, Taube, Čiháková, Wan, Coulombe: "Keratin-dependent regulation of Aire and gene expression in skin tumor keratinocytes." in: Nature genetics, Vol. 47, Issue 8, pp. 933-8, 2015 (PubMed).

Bauch, Koliwer, Buck, Hönck, Kreienkamp: "Subcellular sorting of the G-protein coupled mouse somatostatin receptor 5 by a network of PDZ-domain containing proteins." in: PLoS ONE, Vol. 9, Issue 2, pp. e88529, 2014 (PubMed).

Hagen, Mattay, Räuber, Müller, Arndt: "Characterization and inhibition of AF10-mediated interaction." in: Journal of peptide science : an official publication of the European Peptide Society, Vol. 20, Issue 6, pp. 385-97, 2014 (PubMed).

Mass, Wachten, Aschenbrenner, Voelzmann, Hoch: "Murine Creld1 controls cardiac development through activation of calcineurin/NFATc1 signaling." in: Developmental cell, Vol. 28, Issue 6, pp. 711-26, 2014 (PubMed).

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Yepes, Koch, Waldvogel, Garcia-Betancur, Lopez: "Reconstruction of mreB expression in Staphylococcus aureus via a collection of new integrative plasmids." in: Applied and environmental microbiology, Vol. 80, Issue 13, pp. 3868-78, 2014 (PubMed).

Sechi, Colotti, Belloni, Mattei, Frappaolo, Raffa, Fuller, Giansanti: "GOLPH3 is essential for contractile ring formation and Rab11 localization to the cleavage site during cytokinesis in Drosophila melanogaster." in: PLoS genetics, Vol. 10, Issue 5, pp. e1004305, 2014 (PubMed).

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Stagi, Klein, Gould, Bewersdorf, Strittmatter: "Lysosome size, motility and stress response regulated by fronto-temporal dementia modifier TMEM106B." in: Molecular and cellular neurosciences, Vol. 61, pp. 226-40, 2014 (PubMed).

Köster, Meyer, Weinholdt, Smith, Lummer, Speth, Grosse, Weigel, Staiger: "Regulation of pri-miRNA processing by the hnRNP-like protein AtGRP7 in Arabidopsis." in: Nucleic acids research, Vol. 42, Issue 15, pp. 9925-36, 2014 (PubMed).

Robles Luna, Peña, Borniego, Heinlein, Garcia: "Ophioviruses CPsV and MiLBVV movement protein is encoded in RNA 2 and interacts with the coat protein." in: Virology, Vol. 441, Issue 2, pp. 152-61, 2013 (PubMed).

Ono, Yamada, Endo, Avolio, Lamond: "Analysis of human protein replacement stable cell lines established using snoMEN-PR vector." in: PLoS ONE, Vol. 8, Issue 4, pp. e62305, 2013 (PubMed).

Montesinos, Langhans, Sturm, Hillmer, Aniento, Robinson, Marcote: "Putative p24 complexes in Arabidopsis contain members of the delta and beta subfamilies and cycle in the early secretory pathway." in: Journal of experimental botany, Vol. 64, Issue 11, pp. 3147-67, 2013 (PubMed).

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