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ACAT2 ELISA Kit

ACAT2 Reactivity: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5651338
  • Target See all ACAT2 ELISA Kits
    ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))
    Reactivity
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). No significant cross-reactivity or interference between Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) and analogues was observed.
    Sensitivity
    31.5 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Acetyl Coenzyme A Acetyltransferase 2 (ACAT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Acetyl Coenzyme A Acetyltransferase 2 (ACAT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Acetyl Coenzyme A Acetyltransferase 2 (ACAT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all ACAT2 ELISA Kits
    ACAT2 (Acetyl-CoA Acetyltransferase 2 (ACAT2))
    Alternative Name
    Acetyl Coenzyme A Acetyltransferase 2 (ACAT2 Products)
    Synonyms
    fb10a06 ELISA Kit, fb53f08 ELISA Kit, wu:fb10a06 ELISA Kit, wu:fb53f08 ELISA Kit, AW742799 ELISA Kit, Tcp-1x ELISA Kit, Tcp1-rs1 ELISA Kit, Ab2-076 ELISA Kit, Acat3 ELISA Kit, EMB1276 ELISA Kit, EMBRYO DEFECTIVE 1276 ELISA Kit, MIF21.12 ELISA Kit, MIF21_12 ELISA Kit, acetoacetyl-CoA thiolase 2 ELISA Kit, acetyl-CoA acetyltransferase 2 ELISA Kit, acetyl-Coenzyme A acyltransferase 2 ELISA Kit, acetyl-Coenzyme A acetyltransferase 2 ELISA Kit, acetyl-CoA acetyltransferase 2 S homeolog ELISA Kit, acetoacetyl-CoA thiolase 2 ELISA Kit, ACAT2 ELISA Kit, CNA05060 ELISA Kit, acat2 ELISA Kit, Acat2 ELISA Kit, acat2.S ELISA Kit
    Background

    Gene Name: Acetyl Coenzyme A Acetyltransferase 2

    Gene Aliases: ACTL, Acetoacetyl Coenzyme A Thiolase, Acetyl-CoA transferase-like protein, Cytosolic acetoacetyl-CoA thiolase

    Gene ID
    39
    UniProt
    Q9BWD1
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