Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

A1BG ELISA Kit

A1BG Reactivity: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum
Catalog No. ABIN5651614
  • Target See all A1BG ELISA Kits
    A1BG (alpha-1-B Glycoprotein (A1BG))
    Reactivity
    • 6
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.312 ng/mL - 20 ng/mL
    Minimum Detection Limit
    0.312 ng/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Alpha-1-B-Glycoprotein (a1BG). No significant cross-reactivity or interference between Alpha-1-B-Glycoprotein (a1BG) and analogues was observed.
    Sensitivity
    0.147 ng/mL
    Featured
    Discover our best selling A1BG ELISA Kit
    Top Product
    Discover our top product A1BG ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-1-B-Glycoprotein (a1BG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-1-B-Glycoprotein (a1BG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-1-B-Glycoprotein (a1BG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Alpha-1-B-Glycoprotein (a1BG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-1-B-Glycoprotein (a1BG) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-1-B-Glycoprotein (a1BG) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all A1BG ELISA Kits
    A1BG (alpha-1-B Glycoprotein (A1BG))
    Alternative Name
    Alpha-1-B-Glycoprotein (A1BG Products)
    Synonyms
    A1B ELISA Kit, ABG ELISA Kit, GAB ELISA Kit, HYST2477 ELISA Kit, A1BG ELISA Kit, C44 ELISA Kit, alpha-1-B glycoprotein ELISA Kit, A1BG ELISA Kit, A1bg ELISA Kit
    Background

    Gene Name: Alpha-1-B-Glycoprotein

    Gene ID
    1
    UniProt
    P04217
You are here:
Support