Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

BLMH ELISA Kit

BLMH Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5652234
  • Target See all BLMH ELISA Kits
    BLMH (Bleomycin Hydrolase (BLMH))
    Reactivity
    • 4
    • 2
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.156 ng/mL - 10 ng/mL
    Minimum Detection Limit
    0.156 ng/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Bleomycin Hydrolase (BLMH). No significant cross-reactivity or interference between Bleomycin Hydrolase (BLMH) and analogues was observed.
    Sensitivity
    0.055 ng/mL
    Featured
    Discover our best selling BLMH ELISA Kit
    Top Product
    Discover our top product BLMH ELISA Kit
  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bleomycin Hydrolase (BLMH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bleomycin Hydrolase (BLMH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bleomycin Hydrolase (BLMH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Bleomycin Hydrolase (BLMH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bleomycin Hydrolase (BLMH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all BLMH ELISA Kits
    BLMH (Bleomycin Hydrolase (BLMH))
    Alternative Name
    Bleomycin Hydrolase (BLMH Products)
    Synonyms
    AI035728 ELISA Kit, Bh ELISA Kit, Bmh ELISA Kit, wu:fb13c01 ELISA Kit, zgc:66261 ELISA Kit, bh ELISA Kit, bmh ELISA Kit, BH ELISA Kit, BMH ELISA Kit, bleomycin hydrolase ELISA Kit, Bleomycin hydrolase ELISA Kit, bleomycin hydrolase S homeolog ELISA Kit, BLMH ELISA Kit, pepC ELISA Kit, LOC5569945 ELISA Kit, Apre_0140 ELISA Kit, Apar_0899 ELISA Kit, Smon_1289 ELISA Kit, Kfla_1242 ELISA Kit, Snas_3912 ELISA Kit, BLJ_1941 ELISA Kit, Olsu_1085 ELISA Kit, PGTDC60_0106 ELISA Kit, Blmh ELISA Kit, blmh ELISA Kit, blmh.S ELISA Kit
    Background

    Gene Name: Bleomycin Hydrolase

    Gene Aliases: BH, BMH

    Gene ID
    642
    UniProt
    Q13867
You are here:
Support