CACNA1B ELISA Kit

Catalog No. ABIN5652488

Product Details CACNA1B ELISA Kit

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.312 ng/mL - 20 ng/mL

Minimum Detection Limit: 0.312 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B). No significant cross-reactivity or interference between Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) and analogues was observed.

Sensitivity: 0.129 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNa1B) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CACNA1B

Target: CACNA1B (Calcium Channel, Voltage-Dependent, N Type, alpha 1B Subunit (CACNA1B))

Alternative Name: Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit (CACNA1B Products)

Synonyms: BIII ELISA Kit, CACNL1A5 ELISA Kit, CACNN ELISA Kit, Cav2.2 ELISA Kit, AW050276 ELISA Kit, AW060892 ELISA Kit, AW822256 ELISA Kit, Cchn1a ELISA Kit, alpha(1B) ELISA Kit, calcium voltage-gated channel subunit alpha1 B ELISA Kit, calcium channel, voltage-dependent, N type, alpha 1B subunit ELISA Kit, CACNA1B ELISA Kit, Cacna1b ELISA Kit

Background:

Gene Name: Calcium Channel, Voltage Dependent, N-Type, Alpha 1B Subunit

Gene Aliases: CACNN, CACNL1A5, BIII, Brain calcium channel III, Calcium channel, L type, alpha-1 polypeptide isoform 5, Voltage-gated calcium channel subunit alpha Cav2.2