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CLIC4 ELISA Kit

CLIC4 Reactivity: Human Colorimetric Sandwich ELISA 78.12 pg/mL - 5000 pg/mL Cell Lysate, Tissue Homogenate
Catalog No. ABIN5652873
  • Target See all CLIC4 ELISA Kits
    CLIC4 (Chloride Intracellular Channel 4 (CLIC4))
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    78.12 pg/mL - 5000 pg/mL
    Minimum Detection Limit
    78.12 pg/mL
    Application
    ELISA
    Sample Type
    Cell Lysate, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Chloride Intracellular Channel Protein 4 (CLIC4). No significant cross-reactivity or interference between Chloride Intracellular Channel Protein 4 (CLIC4) and analogues was observed.
    Sensitivity
    30 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chloride Intracellular Channel Protein 4 (CLIC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chloride Intracellular Channel Protein 4 (CLIC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chloride Intracellular Channel Protein 4 (CLIC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chloride Intracellular Channel Protein 4 (CLIC4) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chloride Intracellular Channel Protein 4 (CLIC4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all CLIC4 ELISA Kits
    CLIC4 (Chloride Intracellular Channel 4 (CLIC4))
    Alternative Name
    Chloride Intracellular Channel Protein 4 (CLIC4 Products)
    Synonyms
    CLIC4L ELISA Kit, H1 ELISA Kit, MTCLIC ELISA Kit, huH1 ELISA Kit, p64H1 ELISA Kit, cb142 ELISA Kit, wu:fb33c05 ELISA Kit, wu:fc30h10 ELISA Kit, clic4 ELISA Kit, MGC89006 ELISA Kit, CLIC4 ELISA Kit, DKFZp459A0325 ELISA Kit, D0Jmb3 ELISA Kit, TU-74 ELISA Kit, mc3s5 ELISA Kit, mtCLIC ELISA Kit, xclic4 ELISA Kit, chloride intracellular channel 4 ELISA Kit, chloride intracellular channel 4 (mitochondrial) ELISA Kit, chloride intracellular channel 4 S homeolog ELISA Kit, CLIC4 ELISA Kit, Clic4 ELISA Kit, clic4 ELISA Kit, clic4.S ELISA Kit
    Background

    Gene Name: Chloride Intracellular Channel Protein 4

    Gene Aliases: H1, CLIC4L, huH1, p64H1, Intracellular chloride ion channel protein p64H1

    Gene ID
    25932
    UniProt
    Q9Y696
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