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CH25H ELISA Kit

CH25H Reactivity: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Catalog No. ABIN5652904
  • Target See all CH25H ELISA Kits
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Reactivity
    • 1
    • 1
    • 1
    • 1
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    125 pg/mL - 8000 pg/mL
    Minimum Detection Limit
    125 pg/mL
    Application
    ELISA
    Sample Type
    Plasma, Serum, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificity
    This assay has high sensitivity and excellent specificity for detection of Cholesterol-25-Hydroxylase (CH25H). No significant cross-reactivity or interference between Cholesterol-25-Hydroxylase (CH25H) and analogues was observed.
    Sensitivity
    49 pg/mL
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  • Comment

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Assay Time
    3 h
    Plate
    Pre-coated
    Protocol
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholesterol-25-Hydroxylase (CH25H). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholesterol-25-Hydroxylase (CH25H). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholesterol-25-Hydroxylase (CH25H), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholesterol-25-Hydroxylase (CH25H) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Assay Precision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase (CH25H) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholesterol-25-Hydroxylase (CH25H) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Restrictions
    For Research Use only
  • Handling Advice
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Storage
    4 °C,-20 °C
    Storage Comment
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Expiry Date
    4-8 months
  • Target See all CH25H ELISA Kits
    CH25H (Cholesterol 25-Hydroxylase (CH25H))
    Alternative Name
    Cholesterol-25-Hydroxylase (CH25H Products)
    Synonyms
    CH25H ELISA Kit, zgc:101688 ELISA Kit, MGC143045 ELISA Kit, C25H ELISA Kit, AI462618 ELISA Kit, m25OH ELISA Kit, cholesterol 25-hydroxylase ELISA Kit, cholesterol 25-hydroxylase L homeolog ELISA Kit, CH25H ELISA Kit, ch25h ELISA Kit, Ch25h ELISA Kit, ch25h.L ELISA Kit
    Background

    Gene Name: Cholesterol-25-Hydroxylase

    Gene Aliases: h25OH, Cholesterol 25-monooxygenase

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