F13A1 ELISA Kit

Catalog No. ABIN5653068

Product Details F13A1 ELISA Kit

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Plasma

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Coagulation Factor XIII A1 Polypeptide (F13A1). No significant cross-reactivity or interference between Coagulation Factor XIII A1 Polypeptide (F13A1) and analogues was observed.

Sensitivity: 0.56 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Coagulation Factor XIII A1 Polypeptide (F13A1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Coagulation Factor XIII A1 Polypeptide (F13A1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Coagulation Factor XIII A1 Polypeptide (F13A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Coagulation Factor XIII A1 Polypeptide (F13A1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Coagulation Factor XIII A1 Polypeptide (F13A1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Coagulation Factor XIII A1 Polypeptide (F13A1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Coagulation Factor XIII A1 Polypeptide (F13A1 Products)

Synonyms: F13a ELISA Kit, f13a1 ELISA Kit, wu:fo83d04 ELISA Kit, zgc:153334 ELISA Kit, F13A1 ELISA Kit, F13A ELISA Kit, 1200014I03Rik ELISA Kit, AI462306 ELISA Kit, coagulation factor XIII A1 chain ELISA Kit, coagulation factor XIII, A1 polypeptide b ELISA Kit, coagulation factor XIII A chain ELISA Kit, coagulation factor XIII, A1 subunit ELISA Kit, F13a1 ELISA Kit, f13a1b ELISA Kit, F13A1 ELISA Kit

Background:

Gene Name: Coagulation Factor XIII A1 Polypeptide

Gene Aliases: F13-A1, FXIIIA1, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain, Coagulation factor XIII A chain

Gene ID: 2162

UniProt: P00488