COL1A2 ELISA Kit

Catalog No. ABIN5653103

Product Details COL1A2 ELISA Kit

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62 pg/mL - 1000 pg/mL

Minimum Detection Limit: 15.62 pg/mL

Application: ELISA

Sample Type: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Collagen Type I Alpha 2 (COL1a2). No significant cross-reactivity or interference between Collagen Type I Alpha 2 (COL1a2) and analogues was observed.

Sensitivity: 6.3 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Collagen Type I Alpha 2 (COL1a2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Collagen Type I Alpha 2 (COL1a2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Collagen Type I Alpha 2 (COL1a2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Collagen Type I Alpha 2 (COL1a2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Collagen Type I Alpha 2 (COL1a2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for COL1A2

Target: COL1A2 (Collagen, Type I, alpha 2 (COL1A2))

Alternative Name: Collagen Type I Alpha 2 (COL1A2 Products)

Synonyms: AA960264 ELISA Kit, AI325291 ELISA Kit, Col1a-2 ELISA Kit, Cola-2 ELISA Kit, Cola2 ELISA Kit, oim ELISA Kit, OI4 ELISA Kit, Gas-6 ELISA Kit, alpha2(I) ELISA Kit, hm:zehn2357 ELISA Kit, wu:fa98d05 ELISA Kit, wu:fa99g10 ELISA Kit, wu:fb04c08 ELISA Kit, wu:fb11d06 ELISA Kit, zehn2357 ELISA Kit, coagulation factor II, thrombin ELISA Kit, collagen, type I, alpha 2 ELISA Kit, collagen type I alpha 2 chain ELISA Kit, collagen type I alpha 2 L homeolog ELISA Kit, growth arrest specific 6 ELISA Kit, F2 ELISA Kit, Col1a2 ELISA Kit, col1a2.L ELISA Kit, COL1A2 ELISA Kit, Gas6 ELISA Kit, col1a2 ELISA Kit

Background:

Gene Name: Collagen Type I Alpha 2

Gene Aliases: COL1-A2, OI4, Collagen Alpha-2(I)chain, osteogenesis imperfecta type IV, Collagen Of Skin,Tendon And bone,Alpha-2 Chain

Gene ID: 1278

UniProt: P08123