CKB ELISA Kit

Catalog No. ABIN5653363

Product Details CKB ELISA Kit

Target: CKB (Creatine Kinase, Brain (CKB))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.781 ng/mL - 50 ng/mL

Minimum Detection Limit: 0.781 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Creatine Kinase, Brain (CKB). No significant cross-reactivity or interference between Creatine Kinase, Brain (CKB) and analogues was observed.

Sensitivity: 0.32 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Creatine Kinase, Brain (CKB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Creatine Kinase, Brain (CKB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Creatine Kinase, Brain (CKB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Creatine Kinase, Brain (CKB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Creatine Kinase, Brain (CKB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Creatine Kinase, Brain (CKB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for CKB

Target: CKB (Creatine Kinase, Brain (CKB))

Alternative Name: Creatine Kinase, Brain (CKB Products)

Synonyms: B-CK ELISA Kit, CKBB ELISA Kit, b-ck ELISA Kit, ckbb ELISA Kit, CKB ELISA Kit, Bck ELISA Kit, Ck-3 ELISA Kit, Ck3 ELISA Kit, Ckbb ELISA Kit, bck ELISA Kit, ckb ELISA Kit, cb594 ELISA Kit, wu:fj36f10 ELISA Kit, wu:fj37d11 ELISA Kit, Ckbr ELISA Kit, mg:cb02h12 ELISA Kit, wu:fb62d04 ELISA Kit, wu:fc14g11 ELISA Kit, zgc:154095 ELISA Kit, zgc:92079 ELISA Kit, creatine kinase B ELISA Kit, creatine kinase, brain L homeolog ELISA Kit, creatine kinase B-type ELISA Kit, creatine kinase, brain ELISA Kit, creatine kinase, brain b ELISA Kit, creatine kinase, brain a ELISA Kit, CKB ELISA Kit, ckb.L ELISA Kit, Ckb ELISA Kit, ckb ELISA Kit, ckbb ELISA Kit, ckba ELISA Kit

Background:

Gene Name: Creatine Kinase, Brain

Gene Aliases: CK-BB, CKBB, CK-B, CK-1

Gene ID: 24264

UniProt: P07335