PPIB ELISA Kit

Catalog No. ABIN5653436

Product Details PPIB ELISA Kit

Target: PPIB (Cyclophilin B (PPIB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312 pg/mL - 5000 pg/mL

Minimum Detection Limit: 312 pg/mL

Application: ELISA

Sample Type: Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Cyclophilin B (CYPB). No significant cross-reactivity or interference between Cyclophilin B (CYPB) and analogues was observed.

Sensitivity: 122 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cyclophilin B (CYPB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cyclophilin B (CYPB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cyclophilin B (CYPB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cyclophilin B (CYPB) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cyclophilin B (CYPB) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cyclophilin B (CYPB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for PPIB

Target: PPIB (Cyclophilin B (PPIB))

Alternative Name: Cyclophilin B (PPIB Products)

Synonyms: cypb ELISA Kit, scylp ELISA Kit, cyp-s1 ELISA Kit, PPIB ELISA Kit, Ppib ELISA Kit, ACYPI004891 ELISA Kit, AA408962 ELISA Kit, AA553318 ELISA Kit, AI844835 ELISA Kit, Cphn-2 ELISA Kit, Cphn2 ELISA Kit, CyP-20b ELISA Kit, CypB ELISA Kit, Scylp ELISA Kit, CYP-S1 ELISA Kit, CYPB ELISA Kit, OI9 ELISA Kit, SCYLP ELISA Kit, cb87 ELISA Kit, sb:cb87 ELISA Kit, wu:fa97f08 ELISA Kit, zgc:73214 ELISA Kit, zgc:86796 ELISA Kit, peptidylprolyl isomerase B ELISA Kit, peptidylprolyl isomerase B (cyclophilin B) ELISA Kit, peptidylprolyl isomerase B L homeolog ELISA Kit, ppib ELISA Kit, PPIB ELISA Kit, Ppib ELISA Kit, CC1G_03223 ELISA Kit, ppib.L ELISA Kit

Background:

Gene Name: Cyclophilin B

Gene Aliases: PPIB, PPI-B, CYP-S1, CyP-B, SCYLP, PPIase B, Rotamase B, S-cyclophilin, Peptidylprolyl Isomerase B, Peptidyl-Prolyl Cis-Trans Isomerase B

Gene ID: 5479

UniProt: P23284