DNASE1 ELISA Kit

Catalog No. ABIN5653620

Product Details DNASE1 ELISA Kit

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12 pg/mL - 5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Sample Type: Plasma, Saliva, Seminal Plasma, Serum, Urine

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Deoxyribonuclease I (DNASE1). No significant cross-reactivity or interference between Deoxyribonuclease I (DNASE1) and analogues was observed.

Sensitivity: 30 pg/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Deoxyribonuclease I (DNASE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Deoxyribonuclease I (DNASE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Deoxyribonuclease I (DNASE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Deoxyribonuclease I (DNASE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Deoxyribonuclease I (DNASE1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Deoxyribonuclease I (DNASE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DNASE1

Target: DNASE1 (Deoxyribonuclease I (DNASE1))

Alternative Name: Deoxyribonuclease I (DNASE1 Products)

Synonyms: LOC397802 ELISA Kit, DNL1 ELISA Kit, DRNI ELISA Kit, AI788650 ELISA Kit, DNaseI ELISA Kit, Dnl1 ELISA Kit, dnase1-A ELISA Kit, zgc:92440 ELISA Kit, DNase I ELISA Kit, deoxyribonuclease 1 ELISA Kit, deoxyribonuclease I ELISA Kit, deoxyribonuclease I L homeolog ELISA Kit, Deoxyribonuclease I ELISA Kit, endA ELISA Kit, LOC397802 ELISA Kit, DNASE1 ELISA Kit, Dnase1 ELISA Kit, dnase1.L ELISA Kit, dnase1 ELISA Kit, CpipJ_CPIJ011184 ELISA Kit, CpipJ_CPIJ011185 ELISA Kit, CpipJ_CPIJ011186 ELISA Kit, CpipJ_CPIJ011187 ELISA Kit, CpipJ_CPIJ011190 ELISA Kit, CpipJ_CPIJ017803 ELISA Kit, Desal_1409 ELISA Kit, MPET_RS05255 ELISA Kit, METEV_RS02275 ELISA Kit, Plabr_0631 ELISA Kit

Background:

Gene Name: Deoxyribonuclease I

Gene Aliases: DNaseI, DNL1, DRNI, DNase-I, Dornase alfa

Gene ID: 1773

UniProt: P24855