DARC ELISA Kit

Catalog No. ABIN5653752

Product Details DARC ELISA Kit

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Cell Lysate, Plasma, Serum

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Duffy Blood Group Chemokine Receptor (DARC). No significant cross-reactivity or interference between Duffy Blood Group Chemokine Receptor (DARC) and analogues was observed.

Sensitivity: 0.062 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Duffy Blood Group Chemokine Receptor (DARC). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Duffy Blood Group Chemokine Receptor (DARC). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Duffy Blood Group Chemokine Receptor (DARC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Duffy Blood Group Chemokine Receptor (DARC) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Duffy Blood Group Chemokine Receptor (DARC) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Duffy Blood Group Chemokine Receptor (DARC) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for DARC

Target: DARC (Duffy Blood Group, Chemokine Receptor (DARC))

Alternative Name: Duffy Blood Group Chemokine Receptor (DARC Products)

Synonyms: DARC ELISA Kit, FY ELISA Kit, ACKR1 ELISA Kit, CCBP1 ELISA Kit, CD234 ELISA Kit, Dfy ELISA Kit, GPD ELISA Kit, GpFy ELISA Kit, WBCQ1 ELISA Kit, AA162249 ELISA Kit, Ackr1 ELISA Kit, ESTM35 ELISA Kit, Duffy blood group, chemokine receptor ELISA Kit, atypical chemokine receptor 1 (Duffy blood group) ELISA Kit, atypical chemokine receptor 1 ELISA Kit, DARC ELISA Kit, ACKR1 ELISA Kit, Ackr1 ELISA Kit

Background:

Gene Name: Duffy Blood Group Chemokine Receptor

Gene Aliases: CD234, CCBP1, Dfy, FY, GPD, Duffy Antigen, Ackr1