FBXO32 ELISA Kit

Catalog No. ABIN5654032

Product Details FBXO32 ELISA Kit

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.156 ng/mL - 10 ng/mL

Minimum Detection Limit: 0.156 ng/mL

Application: ELISA

Sample Type: Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of F-Box Protein 32 (FBXO32). No significant cross-reactivity or interference between F-Box Protein 32 (FBXO32) and analogues was observed.

Sensitivity: 0.071 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to F-Box Protein 32 (FBXO32). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to F-Box Protein 32 (FBXO32). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain F-Box Protein 32 (FBXO32), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of F-Box Protein 32 (FBXO32) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level F-Box Protein 32 (FBXO32) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level F-Box Protein 32 (FBXO32) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for FBXO32

Target: FBXO32 (F-Box Protein 32 (FBXO32))

Alternative Name: F-Box Protein 32 (FBXO32 Products)

Synonyms: MGC56479 ELISA Kit, zgc:56479 ELISA Kit, Fbx32 ELISA Kit, MAFbx ELISA Kit, 4833442G10Rik ELISA Kit, AI430017 ELISA Kit, ATROGIN1 ELISA Kit, Gm20361 ELISA Kit, Atrogin1 ELISA Kit, F-box protein 32 ELISA Kit, fbxo32 ELISA Kit, FBXO32 ELISA Kit, Fbxo32 ELISA Kit

Background:

Gene Name: F-Box Protein 32

Gene Aliases: ATROGIN1, Fbx32, MAFbx, Muscle atrophy F-box protein

Gene ID: 114907

UniProt: Q969P5