GAPDH ELISA Kit

Catalog No. ABIN5654734

Product Details GAPDH ELISA Kit

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56 ng/mL - 100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytical Method: Quantitative

Specificity: This assay has high sensitivity and excellent specificity for detection of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). No significant cross-reactivity or interference between Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and analogues was observed.

Sensitivity: 0.63 ng/mL

Application Details

Comment:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Assay Time: 3 h

Plate: Pre-coated

Protocol: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Restrictions: For Research Use only

Handling

Handling Advice: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Storage: 4 °C,-20 °C

Storage Comment: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Expiry Date: 4-8 months

Target Details for GAPDH

Target: GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))

Alternative Name: Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH Products)

Synonyms: G3PD ELISA Kit, GAPD ELISA Kit, Gapd ELISA Kit, BEST:GH12586 ELISA Kit, CG12055 ELISA Kit, Dmel\\CG12055 ELISA Kit, GA3PDH ELISA Kit, GADPH ELISA Kit, GAP ELISA Kit, GAPDH ELISA Kit, GAPDH I ELISA Kit, GAPDH-1 ELISA Kit, GAPDH1 ELISA Kit, GAPDHI ELISA Kit, Gapdh ELISA Kit, Gapdh-1 ELISA Kit, Gapdh43E ELISA Kit, gadph ELISA Kit, gapdh ELISA Kit, gapdh-1 ELISA Kit, gh12586 ELISA Kit, cb609 ELISA Kit, gapd ELISA Kit, mg:bb02e05 ELISA Kit, wu:fb33a10 ELISA Kit, wu:ft80f05 ELISA Kit, KNC-NDS6 ELISA Kit, g3pd ELISA Kit, G3PDH ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase ELISA Kit, Glyceraldehyde 3 phosphate dehydrogenase 1 ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase S homeolog ELISA Kit, glyceraldehyde-3-phosphate dehydrogenase, type I ELISA Kit, olfactory receptor 8K3 ELISA Kit, GAPDH ELISA Kit, Gapdh ELISA Kit, Gapdh1 ELISA Kit, gapdh ELISA Kit, gapdh.S ELISA Kit, gapDH ELISA Kit, LOC100404960 ELISA Kit, LOC614985 ELISA Kit

Background:

Gene Name: Glyceraldehyde-3-Phosphate Dehydrogenase

Gene Aliases: G3PD, GAPD, Peptidyl-cysteine S-nitrosylase GAPDH

Gene ID: 2597

UniProt: P04406